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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20ºC
- 保质期:
1年
- 库存:
30
- 供应商:
上海泽叶生物科技有限公司
- 抗体英文名:
Anti-Mycobacterium tuberculosis, L-alanine dehydrogenase (Ald)
- 规格:
200ug
Anti-Mycobacterium tuberculosis, L-alanine dehydrogenase (Ald)
Bioporto是一家体外诊断公司,专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站:www.bioporto.com
- Cat. No.
- HBT 10
- Product name
- Anti-Mycobacterium tuberculosis, L-alanine dehydrogenase (Ald)
- Description
-
Mouse monoclonal antibody
- Specificity
-
HBT 10 corresponds to WHO no. IT-46 .HBT 10 is specific for a 40 kDa band from short term culture of Mycobacterium tuberculosis in Western blotting after SDS-PAGE. The 40 kDa band was identified as the protein L-alanine dehydrogenase (Ald). Interspecies analyses with HBT 10 showed reacts with M. tuberculosis and M. marinum but not M. bovis BCG (1).
- Immunogen
-
Culture filtrat from M. tuberculosis H37Rv
- Application
- ELISA, WB
- Species reactivity (positive)
- Mycobacterium tuberculosis
- Species reactivity (negative)
- Not determined
- Subclass
-
IgG1/k
- Gene ID
- 888493
- Clone number
- 1C3
- Epitope
-
HBT 10 recognizes the 12 aa SAITDADFKAAG (aa 45-56) region of Ald of M. tuberculosis (2).
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
L-alanine dehydrogenase (Ald) antigen from M. tuberculosis .
- Application 1
-
ELISA
HBT 10 reacts specifically with Ald of M. tuberculosis. Hutter et al (2) has demonstrated that HBT 10 does not react with pyridine nucleotide transhydrogenase (PNT) of Mycobacterium tuberculosis, which has previously been suggested by Delforge et al. (3). - Application 2
-
WB
HBT 10 can be used in immunoblotting, after 1- and 2-dimensional gel electrophoresis (1, 2, 3, 4, 5).
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文献和实验. The released enzymes include creatine kinase, lactate dehydrogenase, and aspartate aminotransferase; the proteins released include troponin T, troponin I, and myoglobin. (See Release of cardiac enzymes and proteins .) Stenosis
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