HBT 10 corresponds to WHO no. IT-46 .HBT 10 is specific for a 40 kDa band from short term culture of Mycobacterium tuberculosis in Western blotting after SDS-PAGE. The 40 kDa band was identified as the protein L-alanine dehydrogenase (Ald). Interspecies analyses with HBT 10 showed reacts with M. tuberculosis and M. marinum but not M. bovis BCG (1).
Immunogen
Culture filtrat from M. tuberculosis H37Rv
Application
ELISA, WB
Species reactivity (positive)
Mycobacterium tuberculosis
Species reactivity (negative)
Not determined
Subclass
IgG1/k
Gene ID
888493
Clone number
1C3
Epitope
HBT 10 recognizes the 12 aa SAITDADFKAAG (aa 45-56) region of Ald of M. tuberculosis (2).
Content
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8ºC without exposure to light. No precautions necessary during handling.
Target
L-alanine dehydrogenase (Ald) antigen from M. tuberculosis .