- $410
- BioPorto
- 丹麦
- HYT 27 (WHO no. IT-49)
- 2025年07月08日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20ºC
- 保质期：
1年
- 库存：
30
- 供应商：
上海泽叶生物科技有限公司
- 抗体英文名：
Anti-Mycobacterium tuberculosis Ag85
- 规格：
200ug
Anti-Mycobacterium tuberculosis Ag85
Bioporto是一家体外诊断公司，专业为临床和科研提供一系列诊断试剂盒和抗体。其领先的产品组合包括了用于急性肾功能损伤诊断用的NGAL以及其它疾病检测用抗体。官方网站：www.bioporto.com
- Cat. No.
- HYT 27 (WHO no. IT-49)
- Product name
- Anti-Mycobacterium tuberculosis Ag85
- Description
-
Mouse monoclonal antibody
- Specificity
-
HYT 27 is specific for Mycobacterium mycolyltransferase. Reacts with M. tuberculosis Ag85C and M. smegmatis Ag85 homolog protein.
No cross-reactivity to antigens from Listeria monocytogenes or Escherichia coli.
- Immunogen
-
Bacterial press extract from M. tuberculosis H37Rv
- Application
- ELISA, WB
- Species reactivity (positive)
- Mycobacterium tuberculosis
- Species reactivity (negative)
- Not determined
- Subclass
-
IgG1/k
- Epitope
-
Epitope is lacking in PPD (culture filtrate, heat treated and precipitated with trichloroacetic acid).
- Content
-
Available in 200 µL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
- Purification
- Protein-A purified
- Form
- Liquid
- Solvent
- 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
- Storage
- 4-8ºC without exposure to light. No precautions necessary during handling.
- Target
-
Mycobacterium tuberculosis antigen 85 is a complex of three related gene products of 30-31 kDa, Ag85A, B and C. All three proteins show fibronectin binding properties and act as mycolyltransferases involved in the final stages of mycobacterial cell wall assembly.
- Application 1
-
ELISA
HYT 27 reacts strongly in ELISA with M. tuberculosis cell culture filtrate or whole cells directly coated onto the microtiter wells (1). - Application 2
-
WB
In Western blotting after SDS-PAGE, HYT 27 reacts strongly with Ag85C and weaker with Ag85A and Ag85B.
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文献和实验The Mycobacterium tuberculosis Phagosome
Tuberculosis is currently the most devastating human bacterial disease, causing millions of deaths annually and infecting an overwhelming percentage of the global population. Its success as a scourge lies in the ability of Mycobacterium
Analysis of the Capsule of Mycobacterium tuberculosis
Mycobacterium tuberculosis and the other pathogenic mycobacteria examined so far by electron microscopy are seen to be surrounded within host cells by an electron-transparent zone (ETZ). For Mycobacterium lepraemurium , this space
Analysis of the Lipids of Mycobacterium tuberculosis
Mycobacterial cell wall ultrastructure has been studied through the use of negative staining, electron microscopy (1 ,2 ), freeze fracture (3 ), X-ray diffraction (4 ), differential scanning calorimetry (5 ,6 ), and electron spin
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