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- 详细信息
- 文献和实验
- 技术资料
- 英文名：
5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set
- 库存：
大量
- 供应商：
简石生物
- 保存条件：
-70C
HIGHLIGHTS
- Control DNA for 5-methylcytosine and 5-hydroxymethylcytosine quantitation applications (ex. mass spectrometry, HPLC, TLC, etc.)
- Substrate for studies involving methylated cytosine and interacting proteins
DESCRIPTION
The 5-Methylcytosine & 5-Hydroxymethylcytosine DNA Standard Set is a set of three standards that are linear dsDNA at a length of 897 bp. All DNA standards have the same sequence (see protocol for sequence), the only difference being that each standard contains either 100% unmodified cytosines, 5-methylcytosines, or 5-hydroxymethylcytosines. Since the sequence and extent of cytosine modification is known, this DNA standard set is ideal for use in calibration of various applications intended for quantitation of cytosine modifications.
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文献和实验strands [J]. Proc Natl Acad Sci USA, 1992, 89: 1827–1831.[26]朱燕. DNA的甲基化的分析与状态检测 [J]. 现代预防医学, 2005, 32(9): 1070-1073.[27]沈佳尧, 侯鹏, 祭美菊等. DNA甲基化方法研究现状 [J]. 生命的化学, 2003, 23(2): 149-151.[28]Herman J G, Graff J R, Myohanen S, et al. Methylation-specific PCR
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
dihydrochloride Kodak #X8748 DNA PAGE % gel 3.5 5.0 8.0 12.0 20.0 30% acryl stock 11.6 16.6 26.6 40.0 66
Standard hybridization technique
on that given in Maniatis et al.,(1982)with both Denhart's solution and heterologous DNA being replaced by heparin (Singh and Jones,1984). You will need: 6 x SSC (0.9M NaCl,90mM sodium citrate,pH 7) 10% SDS 50mg/ml heparin
