Recombinant Mouse IL-4

Recombinant Mouse IL-4  (CK74)

产品说明(Description)

Recombinant Mouse Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His21-Ser140 is expressed with a 6His tag at the C-terminus. 

Accession #: P07750

Known as: Interleukin-4; IL-4; IL4; B-cell IgG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1; BSF-1; IGG1 induction factor; Lymphocyte stimulatory factor 1

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

 

质量控制(Quality Control)

Purity:           Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity-ELISA:		Bioactivity-BLI:
Measured in a cell proliferation assay using M-NFS-60 mouse lymphoblast cells. The ED50 for this effect is 0.035 ng/mL.		Loaded Mouse IL-4-His(Cat#CK74) on HIS1K Biosensor, can bind Mouse IL-4RA-Fc(Cat#CB03) with an affinity constant of <10-3 nM as determined in BLI assay.

 

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

BFAR coordinates TGFβ signaling to modulate Th9-mediated cancer immunotherapy[J]. Siyu Pei. et al. Journal of Experimental Medicine. 2021.

Human amniotic mesenchymal stem cells-conditioned medium protects mice from high-fat diet-induced obesity[J]. Hui-Lan Tan. et al. Stem Cell Research & Therapy. 2021.

Bmal1 Regulates Macrophage Polarize Through Glycolytic Pathway in Alcoholic Liver Disease[J]. Yiwen Zhou. et al. Frontiers in Pharmacology. 2021.

A DNA nanodevice-based vaccine for cancer immunotherapy[J]. Shaoli Liu. et al. nature materials. 2020.

An interleukin-4-loaded bi-layer 3D printed scaffold promotes osteochondral regeneration[J]. Lin Gong.et al. Acta Biomaterialia. 2020.

Critical roles of conventional dendritic cells in autoimmune hepatitis via autophagy regulation[J]. Xiaoli Fan. et al. Cell Death & Disease. 2020.

Total glucosides of paeony decreases apoptosis of hepatocytes and inhibits maturation of dendritic cells in autoimmune hepatitis[J]. Mengyi Shen. et al. BIOMEDICINE & PHARMACOTHERAPY. 2020.

A Recombinant Rabies Virus Expressing Fms-like Tyrosine Kinase 3 Ligand (Flt3L) Induces Enhanced Immunogenicity in Mice[J]. Yachun Zhang. et al. Virologica Sinica. 2019.

Deficiency of Complement C3a and C5a Receptors Prevents Angiotensin II-Induced Hypertension via Regulatory T Cells[J]. Xiao Hui Chen. et al. Circulation Research. 2018.

The mTOR–S6K pathway links growth signalling to DNA damage response by targeting RNF168[J]. Xiaoduo Xie. et al. nature cell biology. 2018.

 

 

FOR RESEARCH USE ONLY