Recombinant Mouse/Rat TGF-beta

Recombinant Mouse/Rat TGF-beta 1 (CK33)

产品说明(Description)

Recombinant Mouse/Rat Transforming Growth Factor beta 1 is produced by our Mammalian expression system and the target gene encoding Ala279-Ser390 is expressed. 

Accession #: P04202

Known as: TGF-beta-1; CED; DPD1; TGFB; TGF-b1; TGFB1; CEDLAP;latency-associated peptide; TGFbeta; TGF-beta 1 protein; transforming growth factor beta-1; TGF-β1; TGF beta1; TGFbeta 1; TGF-beta 1; TGFbeta

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 4mM Hcl.

 

质量控制(Quality Control) 

Purity:          Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:   Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity:  Measured by its ability to inhibit IL-4-dependent proliferation of TF-1 human erythroleukemic cells.

                      The ED50 for this effect is 5-25 pg/ml.

 

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 4mM Hcl.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Transforming growth factor beta 1 (TGFβ1) is the prototype of a growing superfamily of peptide growth factors and plays a prominent role in a variety of cellular processes, including cell-cycle progression, cell differentiation, reproductive function, development, motility, adhesion, neuronal growth, bone morphogenesis, wound healing, and immune surveillance. TGF-β1, TGF-β2 and TGF-β3 signal via the same heteromeric receptor complex, consisting of a ligand binding TGF-β receptor type II (TβR-II), and a TGF-β receptor type I (TβR-I). Signal transduction from the receptor to the nucleus is mediated via SMADs. TGF-β expression is found in cartilage, bone, teeth, muscle, heart, blood vessels, haematopoitic cells, lung, kidney, gut, liver, eye, ear, skin, and the nervous system.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

Inhibition of TGFβ1 accelerates regeneration of fibrotic rat liver elicited by a novel two-staged hepatectomy[J]. Bo Zhang. et al. Theranostics. 2021.

Salt Sensing by Serum/Glucocorticoid-Regulated Kinase 1 Promotes Th17-like Inflammatory Adaptation of Foxp3+ Regulatory T Cells[J]. Yujian H. Yang. et al. Cell Reports. 2020.

Overexpression of PTPRN Promotes Metastasis of Lung Adenocarcinoma and Suppresses NK Cell Cytotoxicity[J]. Xinyue Song. et al. Frontiers in Cell and Developmental Biology. 2021.

 

TGF-β1 Induces Immune Escape by Enhancing PD-1 and CTLA-4 Expression on T Lymphocytes in Hepatocellular Carcinoma[J]. Shixiang Bao. et al. Frontiers in Oncology. 2021.

CD4+ T-Cell Differentiation In Vitro[J]. Wenyong Yang. et al. Methods in Molecular Biology. 2020.

Glycoprotein M6B Interacts with TβRI to Activate TGF‐β‐Smad2/3 Signaling and Promote Smooth Muscle Cell Differentiation[J]. Xiaomeng Zhang. et al. Stem Cells. 2018.

CD4+ T-Cell Differentiation In Vitro[J]. Wenyong Yang. et al. Methods in Molecular Biology. 2020.

Natural product mogrol attenuates bleomycin-induced pulmonary fibrosis development through promoting AMPK activation[J]. Bei Liu. et al. Journal of Functional Foods. 2021.

miR-130a/TGF-β1 axis is involved in sow fertility by controlling granulosa cell apoptosis[J]. Xing Du.et al. Theriogenology. 2020.

The canonical TGF-β/Smad signalling pathway is involved in PD-L1-induced primary resistance to EGFR-TKIs in EGFR-mutant non-small-cell lung cancer[J]. Yang Zhang. et al. Respiratory Research.2019.

T RII Regulates the Proliferation of Metanephric Mesenchyme Cells through Six2 In Vitro. Zhaomin Mao. et al. International Journal of Molecular Sciences. 2017.

 

 

FOR RESEARCH USE ONLY