Recombinant Human TRANCE/RANK

Recombinant Human TRANCE/RANK L/TNFSF11/CD254  (CK63)

 

产品说明(Description)

Recombinant Human Receptor Activator of NF-kappa-B ligand is produced by our E.coli expression system and the target gene encoding Ile140-Asp317 is expressed. 

Accession #: O14788

Known as: CD254; ODF; OPGL; RANKL; TNFSF11; CD254; Osteoclast differentiation factor;  Receptor activator of nuclear factor kappa-B ligand; tumor necrosis factor ligand superfamily member 11

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 20mM Tris,150mM NaCl,pH8.0.

 

质量控制（Quality Control） 

Purity:           Greater than 90% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity:    Immobilized ZRKLA (Cat#CK63) at 2μg/ml (100 μl/well) can bind Human OPG-His (Cat#C325)

                       The ED50 of ZRKLA (Cat#CK63) is  0.15 ug/ml .

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

CD254, also known as RANKL, TNFSF11, TRANCE, OPGL and ODF, is a type II membrane protein of the tumor necrosis factor (TNF) superfamily, and affects the immune system and control bone regeneration and remodeling. RANKL is the ligand of nuclear factor (NF)-κB (RANK). When RANKL binds to RANK, it will undergo trimerization and then bind to an adaptor molecule TNF receptor-associated factor 6 (TRAF6). This results in the activation of several downstream signaling cascades, including the NFκB, mitogen-activated protein kinases (MAPK), activating protein 1 (AP-1), and nuclear factor of activated T cells (NFATc1), resulting in the formation of multinucleated bone-resorbing osteoclasts. RANKL is widely expressed in skeletal muscle, thymus, liver, colon, small intestine, adrenal gland, osteoblast, mammary gland epithelial cells, prostate and pancreas.

 

电泳(SDS-PAGE)

 

参考文献（References）

Development of Small-Molecules Targeting Receptor Activator of Nuclear Factor-κB Ligand (RANKL)—Receptor Activator of Nuclear Factor-κB (RANK) Protein–Protein Interaction by Structure-Based Virtual Screening and Hit Optimization[J]. Min Jiang. et al. Journal of Medicinal Chemistry. 2019.

 

FOR RESEARCH USE ONLY