Recombinant Mouse GM-CSF

Recombinant Mouse GM-CSF  (CJ46)

 

产品说明(Description)

Recombinant Mouse Granulocyte-Macrophage Colony-Stimulating Factor is produced by our Mammalian expression system and the target gene encoding Ala18-Lys141 is expressed with a 6His tag at the C-terminus. 

Accession #: P01587

Known as: Granulocyte-Macrophage Colony-Stimulating Factor; GM-CSF; Colony-Stimulating Factor; CSF;Molgramostin; Sargramostim; CSF2; GMCSF

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of PBS,  pH 7.4.

 

质量控制（Quality Control） 

Purity:             Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:      Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity:  Measured in a cell proliferation assay using FDC-P1 cells.

                          The ED50 for this effect is 40-170 pg/ml.

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

Granulocyte-Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factorthat can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by anumber of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cellsand fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophageprogenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. Onmature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has a functional role on nonhematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF canalso stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma andadenocarcinoma cell lines.

 

电泳(SDS-PAGE)

 

参考文献（References）

HIV-2/SIV Vpx targets a novel functional domain of STING to selectively inhibit cGAS–STING-mediated NF-κB signalling[J]. Jiaming Su. et al. Nature Microbiology. 2019.

Prim-O-glucosylcimifugin enhances the antitumour effect of PD-1 inhibition by targeting myeloid-derived suppressor cells[J]. Wanfeng Gao. et al. Journal for ImmunoTherapy of Cancer. 2019.

 

FOR RESEARCH USE ONLY