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文献和实验Fluorochrome Absorption and Emission
daltons and an absorption maximum at 495 nm (Fig. 2). Its excitation by 488-nm light leads to a fluorescence emission maximum around 520 nm (Fig. 2). Using a 530 ?15 nm bandpass (BP) filter will give optimum detection for this fluorochrome
Filter Binding Assay for EBNA-1
apparatus and incubate at filter binding temperature (4C) for 10min. Pass through filter as above and collect in a labeled tube.Load 400ul Wash Buffer into the syringe. Reattach the plunger and wash the filter immediately, collecting fraction in a separate
Fluorochrome Absorption and Emission Spectra
daltons and an absorption maximum at 495 nm (Fig. 2). Its excitation by 488-nm light leads to a fluorescence emission maximum around 520 nm (Fig. 2). Using a 530 ?15 nm bandpass (BP) filter will give optimum detection for this fluorochrome
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