PMA/TPA (PKC激活剂)

Phorbol 12-myristate 13-acetate (PMA) is a phorbol ester that is commonly used to activate certain types of protein kinase C (PKC), including group A (α, βI, βII, γ) and group B (δ, ε, η, θ) isoforms. Phorbol esters, including PMA, are structurally analogous to diacylglycerol and activate PKC isoforms by associating with their C1 domains.Through PKC, PMA also activates certain MAP kinase pathways.Prolonged treatment of cells with PMA at high concentrations results in the downregulation of total PKC activity and is tumorigenic, whereas lower concentrations may be protective. In addition, PMA promotes hematopoietic differentiation. The aqueous solubility of PMA is about 10-fold better than that of phorbol-12,13-dibutyrate, indicating that PMA is preferable in cell-based studies.