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100 µg
蛋白名称:Ephrin A5蛋白, Ephrin A5 protein
蛋白构建:A DNA sequence encoding the mouse EFNA5 isoform long (O08543-1) without the pro peptide (Met 1-Asn 203) was as fused with the Fc region of human IgG1 at the C-terminus.
表达宿主:HEK293 Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:Measured by its binding ability in a functional ELISA . Immobilized Mouse EPHA4 at 2 μg/ml (100 μl/well) can bind mouse EFNA5-Fc with a linear ranger of 1.28-32 ng/ml.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Gln 21
蛋白分子量:The recombinant mouse EFNA5/Fc is a disulfide-linked homodimer. The reduced monomer consists of 424 amino acids and has a predicted molecular mass of 48.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmEFNA5/Fc monomer is approximately 52 kDa due to glycosylation.
蛋白NP号:O08543-1
蛋白氨基酸序列:Met1-Asn203
蛋白标签:C-human IgG1-Fc
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 人载脂蛋白A5 (APO A5)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和其它生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗人 APO A5 单抗包被于酶标板上,标准品和样品中的 APO A5与单抗结合,加入生物素化的抗人 APO
大鼠 载脂蛋白A5(APO-A5) 酶联免疫 分析( ELISA ) 试剂 盒使用说明书 本试剂仅供研究使用 目的:本试剂盒用于测定大鼠血清,血浆及相关液体样本中载脂蛋白A5(APO-A5)含量。 实验原理 : 本试剂盒应用双抗体夹心法测定 标本 中 大鼠载脂蛋白A5 ( APO-A5 ) 水平。用纯化的 大鼠载脂蛋白A5 ( APO-A5 ) 抗体包被微孔板,制成固相抗体,往包被单抗的微孔中依次加入 载脂蛋白A5 ( APO-A5
therapeutic compounds that promote axon regeneration. Here, we describe a growth cone collapse assay protocol used to study signal transduction mechanisms of the repulsive axon guidance molecule ephrin-A5 in hippocampal neurons.
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