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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human CDC37 / CDC37A Protein (GST Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
50 µg
蛋白名称:CDC37蛋白, CDC37 protein
蛋白构建:A DNA sequence encoding the full length of human CDC37 (NP_008996.1) (Met 1-Val 378) was fused with the GST tag at the C-terminus.
表达宿主:Baculovirus-Insect Cells
蛋白纯度:> 85 % as determined by SDS-PAGE
蛋白活性:0
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Met
蛋白分子量:The recombinant human CDC37/GST chimera consists of 603 amino acids and has a predicted a molecular mass of 70.7 kDa as estimated in SDS-PAGE under reducing conditions.
蛋白NP号:NP_008996.1
蛋白氨基酸序列:Met1-Val378
蛋白标签:N-GST
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Huang, W; et al. FW-04-806 inhibits proliferation and induces apoptosis in human breast cancer cells by binding to N-terminus of Hsp90 and disrupting Hsp90-Cdc37 complex formation. Mol. Cancer, Pubmed: 24927996
Preparation of Recombinant Protein Spotted Arrays for Proteome‐Wide Identification of Kinase Targets
, H. 2009. Profiling the human protein‐DNA interactome reveals ERK2 as a transcriptional repressor of interferon signaling. Cell 139:610‐622. Hudson, J.R., Jr., Dawson, E.P
GST融合蛋白的准备 Preparation of Glutathione-S-Transferase (GST) Fusion Proteins
REFERENCES Cox, G.N., Pratt D., McDermott M.J. and van der Slice R.W. 1999. Refolding and characterization of recombinant human soluble CTLA-4 expressed in E. coli. Protein Expr. Purif . 17 : 26-32. Frangoni J
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
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