Phospho-IKKα/β (Ser176/180) (1

6A6) Rabbit mAb

价格询价

品牌Cell Signaling Technology
货号
2697
产地USA
更新日期2026年05月28日

应用范围W, IHC-P, IHC-F
适应物种H,M,R,Mk,B

美国CST中国公司(丁香通商铺)

品牌商

13 年钻石会员

详细信息
文献和实验
技术资料

抗体英文名：
Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb
抗原：
synthetic phosphopeptide corresponding to residues surrounding Ser176/180 of human IKKα
应用范围：
W, IHC-P, IHC-F
供应商：
CST
适应物种：
H,M,R,Mk,B
级别：
详见MSDS文件
保质期：
详见说明书
库存：
大量
是否单克隆：
1
保存条件：
-20°c
规格：
40 ul (4 western blots)/100 ul (10 western blots)/300 ul (30 western blots)/carrier free & custom formulation / quantity

规格：		产品价格：	￥请询价
规格：	40 ul (4 western blots)	产品价格：	￥请询价
规格：	100 ul (10 western blots)	产品价格：	￥请询价
规格：	300 ul (30 western blots)	产品价格：	￥请询价
规格：	carrier free & custom formulation / quantity	产品价格：	￥请询价

pathway more info application references datasheet PDF MSDS PDF protocols

Applications Key: W=Western Blotting IHC-P=Immunohistochemistry (Paraffin) IHC-F=Immunohistochemistry (Frozen)
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey B=Bovine
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IHC-P IHC-F	H M R Mk (B)	Endogenous	85 IKK-alpha 87 IKK-beta	Rabbit IgG

Protocols	2697: IHC / Frozen , IHC / Paraffin , Western Blotting
Specificity / Sensitivity	Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb detects IKKα only when phosphorylated at Ser176/180 and IKKβ only when phosphorylated at Ser177/181.
Source / Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser176/180 of human IKKα. Western Blotting Western blot analysis of extracts from THP-1 cells, differentiated with TPA (#9905, 80 nM for 24h) and treated with 1 μg/ml LPS for the indicated times, using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb. Western Blotting Western blot analysis of extracts from TNF-alpha and Calyculin A treated HeLa and NIH/3T3 cells, using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human gall bladder (chronic cholecystitis), using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human colon carcinoma, untreated (left) or λ phosphatase-treated (right), using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human colon carcinoma, showing cytoplasmic localization, using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human lung (chronic bronchitis), using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb. IHC-P (paraffin) Immunohistochemical analysis of paraffin-embedded human breast carcinoma, using Phospho-IKKα/β (Ser176/180) (16A6) Rabbit mAb in the presence of control peptide (left) or Phospho-IKK-alpha/beta (Ser176/180) Blocking Peptide #1023 (right). IHC-F (frozen) Immunohistochemical analysis of frozen H1650 xenograft, showing cytoplasmic localization using Phospho-IKKα/β (Ser176/180)(16A6) Rabbit mAb.
Background	The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins (1-3). Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB (3-7). The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit (8,9). Activation of IKK depends upon phosphorylation of Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes resulting in kinase activation (10-13). Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8. Brown, K. et al. (1995) Science 267, 1485-8. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83. Chen, Z.J. et al. (1996) Cell 84, 853-62. Zandi, E. et al. (1997) Cell 91, 243-52. Karin, M. (1999) Oncogene 18, 6867-74. DiDonato, J.A. et al. (1997) Nature 388, 548-54. Mercurio, F. et al. (1997) Science 278, 860-6. Johnson, L.N. et al. (1996) Cell 85, 149-58. Delhase, M. et al. (1999) Science 284, 309-13.
Application References	Kwong, C. et al. (2011) J Immunol 186, 1781-9. Applications: Western Blotting Barisic, S. et al. (2010) Biochem Pharmacol 80, 439-47. Applications: Western Blotting Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know !
Companion Products	2370 IKKβ (2C8) Rabbit mAb 2682 IKKα Antibody 2078 Phospho-IKKα (Ser176)/IKKβ (Ser177) (C84E11) Rabbit mAb 2694 Phospho-IKKα/β (Ser176/180) Antibody II 2684 IKKβ Antibody 2678 IKKβ (L570) Antibody 1023 Phospho-IKK-alpha/beta (Ser176/180) Blocking Peptide 7071 Phototope^® -HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody 7074 Anti-rabbit IgG, HRP-linked Antibody 7720 Prestained Protein Marker, Broad Range (Premixed Format) 7727 Biotinylated Protein Ladder Detection Pack 7003 20X LumiGLO^® Reagent and 20X Peroxide 8114 SignalStain^® Boost IHC Detection Reagent (HRP, Rabbit) 9997 Tris Buffered Saline with Tween 20 (TBST-10X) 5425 Normal Goat Serum Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063. For Research Use Only. Not For Use In Diagnostic Procedures.