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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
干冰运输,-80℃保存
- 保质期:
6个月
- 英文名:
Top10 Competent Cell
- 库存:
大量
- 供应商:
上海钰博生物科技有限公司
- 规格:
0.1 mL×10/盒
Top10感受态细胞产品及特点
本菌种最大特点是高转化效率,其它特点如下:
干冰运输,-80℃保存,有效期6个月。Taq DNA Polymerase(500U)
Taq DNA Polymerase(1000U)
Taq DNA Polymerase(5000U)
Taq DNA Polymerase(10000U)
2 X Taq MasterMixture(5x1ml)不含染料
2 X Taq MasterMixture(20x1ml)不含染料
2 X Taq MasterMixture(50x1ml)不含染料
2 X Taq MasterMixture(5x1ml)含染料
2 X Taq MasterMixture(20x1ml)含染料
2 X Taq MasterMixture(50x1ml)含染料
dNTP solution,10mM(4x250ul)
dNTP solution,25mM(4x250ul)
dATP solution,100mM(250ul)
dcTP solution,100mM(250ul)
dGTP solution,100mM(250ul)
dTTP solution,100mM(250ul)
Tissue Direct PCR Kit(20)
Tissue Direct PCR Kit(100)
Tissue Direct PCR Kit(500)
Blood Direct PCR Kit(20)
Blood Direct PCR Kit(100)
Blood Direct PCR Kit(500)
100bp DNA Ladder(50)
100bp DNA Ladder(200)
200bp DNA ladder(50)
200bp DNA Ladder(200)
1kbp DNA Ladder(50)
1kbp DNA Ladder(200)
HT Ladder I(50)
HT Ladder I(200)
HT Ladder II (50)
HT Ladder II (200)
CL5000 Ladder (50)
CL5000 Ladder (200)
EZ 96 Vacuum Manifolds
Vacuum Manifolds
E-Z 96 Magnetic Separation Device-A
E-Z 96 Magnetic Separation Device-B
Magnetic Separation Device-B
96 well 2ml collection plate(5)
96 well 2ml collection plate(25)
96 well 500ul collection plate(5)
96 well 500ul collection plate(25)
1.2ml Round Well Plate(10)
1.2ml Round Well Plate(100)
1.2ml 8-Strip Caps,Non-strerile,125 strips
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文献和实验TOP10 chemically competent cells
Detergent is a major inhibitor of competent cell growth and transformation. Glass and plastic must be detergent free for these protocols. The easiest way to do this is to avoid washing glassware, and simply rinse it out. Autoclaving glassware filled
CCMB buffer to make chemical competent cell
TOP10 chemically competent cells ( modified from http://openwetware.org/wiki/TOP10_chemically_competent_cells#CCMB80_buffer) I also use this protocol to make Ecoli compent cell. It is pretty good. You can start from the fresh clone on plate
实验概要 Competent cells are those that possess more easily altered cell walls that DNA can be passed through easily. These cells readily incorporate foreign DNA. On example of a competent cell is E. coli.
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