RP/HILIC UPLC Method Developme

Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit

the medium, and re-suspend the cells in an appropriate amount of fibroblast medium.         d. Count the cells using the desired method (e.g., Countess® Automated Cell Counter), and seed the MEF culture dishes with 5 × 104 –2 × 105 cells per 100-mm dish

E.Z.N.A.® Total RNA Midi Kit Protocol for Eukaryotic Cells and Tissues

or 50 ml centrifuge tube and proceed to step 2 below. (This method is preferable to trypsinization followed by washing because it minimizes RNA degradation by nuclease contamination.) For cells grown in suspension cultures, pellet cells

新型光机结合的二维共焦生物芯片扫描仪设计

扫描仪中，都采用机械式的二维X，Y线性扫描技术实现，即X，Y方向都采用直线驱动器和直线导轨实现往复运动。此类装置，由于驱动系统的频率限制，驱动器的扫描惯性大，使得扫描效率低，分析时间相当长；并且往复行程长，对直线导轨的精度要求相当高。二、光机结合的二维扫描系统为同样实现生物芯片的二维扫描，我们的实验装置设计如图2，采用了振镜和大数值孔径的远心f-è物镜相结合实现X方向扫描，Y方向的运动仍采用直线驱动器和直线导轨实现。 系统中，对于f-è物镜，满足x＝2fè（è为振镜的摆动角度，f为物镜焦距）的线性