Isolator Column

DEAE Column

on the rotary shaker (in cold room)at a speed of 3 for a period of 40 mins. 8)Load gel onto column and collect flow through. 9)Wash column with 5 column volumes of starting buffer. - In actuality,a more rigorous manner in which to check washing is to monitor

合蕊柱column，gynostemium

  　　主要为兰科（ Orchidaceae）植物花中的雌蕊和雄蕊互相愈合所成的器官。多为柱状，顶端着生着一个花药，有时该部分形成凹陷，称为药窝，接近顶端处形成柱头。在柱头和雄蕊之间有蕊喙，药窝上面横载着由药内花粉块伸长而成的花粉块柄。 　　蕊喙具有弹性，当昆虫飞来吸蜜而把头部钻进花腔时，蕊喙就弯曲过来，于是花粉块柄顶端的着粉盘露出，接触于昆虫体上。在合蕊柱的基部，通常着生着唇瓣，但其前面向前下方突出，顶端接着于唇瓣，因此外观上呈下颚状

Spin Column chromatography

-50 (medium) by adding appropriate amount of dry beads to 100 ml TE buffer such that the beads will swell to 50 ml. Autoclave. 1. Rock bottle gently to make a uniform slurry. 2. Place a disposable 3ml chromatography column into a 15 ml