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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
- 英文名:
TBP (TATA-box binding protein) Gene Endogenous Control Kit
- 规格:
P4252-200UN
TBP (TATA-box binding protein) Endogenous Control Kit |
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| 目录号 |
规格 |
价格 |
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| QPG-03090 |
100 rxns |
¥1,000 |
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| QPG-03091 |
200 rxns |
¥1,500 |
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| QPG-03092 |
300rxns |
¥2,200 |
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| QPG-03093 |
500 rxns |
¥3,000 |
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| 试剂盒组成(40μl 反应体系×100次)
Real-Time PCR Master Mix(5×Conc.)* 1.0 ml×1支 Taq DNA polymerase (5U/μl) 40μl×1支 GAPDH RT Primer (50μM) 50μl×1支 dd H2 O 1ml×4支 *内含dNTP Mixture, Mg2+ , PCR引物,GAPDH荧光探针等。 |
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□产品特点 |
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| Ø 预先优化好的荧光定量PCR反应体系; Ø 应用TaqMan荧光PCR基因检测技术检测样本中TBP 基因; Ø 实验操作简便,只需加入模板和Taq 酶即可。 Ø 可直接用于以GAPDH为内参照的基因表达定量研究。
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| □适用的荧光定量PCR仪器 | |||||||
| ABI PRISM 7000/7300/7500/7900 MJ-opticon、Opticon2及Chromo4 系列 Bio-Rad IQ系列、Light Cycler、Rotogen 3000 MX3000P/4000、FTC-2000
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文献和实验Purification of the human TATA-Binding Protein, TBP
The TATA-binding protein TBP plays a central role in eukaryotic transcription initiation (1 , 2 ). TBP binds to the promoter consensus sequence TATAAA, located approx 30 nucleotides upstream of the transcriptional start site. Once bound, TBP
Using FRET to Monitor Protein-Induced DNA Bending: The TBP-TATA Complex as a Model System
Proteins that bind to DNA can elicit changes in DNA conformation, such as bending and looping, which are important signals for later events such as transcription. TATA-binding protein (TBP) is one example of a protein that elicits
适合从动物细胞、动物组织或酵母菌中同步纯化总RNA 和基因组DNA纯化的总RNA ,适合用于Northern Blot、RT-PCR、体外翻译、Primer Extension、S1 核酸酶作图、RNase 保护测定、制备mRNA 和构建cDNA 文库等各种RNA 分子生物学实验。纯化的基因组DNA 适用于PCR、Southern Blot、RAPD、AFLP、RFLP 等分子生物学实验。一、试剂盒组成、贮存、稳定性以每次从1×107 动物细胞、100mg 动物组织或5×107 酵母菌中
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