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- 文献和实验
- 技术资料
- 规格:
100ul/1ml
| 规格: | 100ul | 产品价格: | ¥180.0 |
|---|---|---|---|
| 规格: | 1ml | 产品价格: | ¥1200.0 |
| 产品编号 | bs-0297G-HRP |
| 英文名称 | Goat Anti-Human IgG H&L, HRP conjugated |
| 中文名称 | 辣根过氧化物酶标记的羊抗人IgG H&L |
| 英文别名 | Goat Anti-Human IgG H&L (HRP); Immunoglobulin G; |
| 产品应用 | WB=1:2000-10000, IHC-P=1:100-500, IHC-F=1:100-1000, ELISA=1:1000-10000 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Goat |
| 免疫原 | Native Human IgG |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein G, nonspecific adsorbed |
| 克隆类型 | Polyclonal |
| 浓度 | 2.0 mg/ml |
| 储存液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants.br> |
| 应用 | 推荐稀释比例 |
| {WB} | {1:2000-10000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-1000} |
| {ELISA} | {1:1000-10000} |

Lane 1,2: Human IgG (reduced form)
Lane 3,4: Human IgG (non reduced form)
Primary: Goat Anti-Human IgG H&L(HRP) (bs-0297G-HRP) at 1/2000 dilution
Predicted band size: 50,25,150 kD
Observed band size: 50,25,150 kD
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文献和实验我是用来做筛选IgG型单克隆抗体,用它做二抗,筛到的阳性克隆化和扩大培养后用SIGMA亚类试剂盒鉴定却是IgM,郁闷!!!这个试剂盒能与IGM交叉?是不是抗轻链?我这种方式不对吗?谁知道有较好的抗鼠IgG而与IgM不交叉的抗体?? 试剂盒说明书:Anti-mouse IgG-HRP Antibody FOR RESEARCH USE ONLY. NOT FOR USE IN HUMANS. R&D Systems, Inc. 1-800-343-7475 2/21/06
Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
which employs goat anti-Rabbit HRP.12. Wash filter 3 times 7 minutes with 0.5% BSA in TBST.13. Perform Alkaline phosphatase reaction using NBT and BCIP substrates. I find that CREB usually comes up within several minutes of adding the AP substrates
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