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- 文献和实验
- 技术资料
- 规格:
1mg/10mg
| 规格: | 1mg | 产品价格: | ¥980.0 |
|---|---|---|---|
| 规格: | 10mg | 产品价格: | ¥4800.0 |
| 产品编号 | bs-0297G |
| 英文名称 | Goat Anti-Human IgG H&L |
| 中文名称 | 羊抗人IgG H&L |
| 英文别名 | Human Immunoglobulin G; hu IgG; |
| 交叉反应 | Human |
| 抗体来源 | Goat |
| 免疫原 | Native Human IgG |
| 亚型 | IgG |
| 性状 | Lyophilized or Liqui |
| 纯化方法 | affinity purified by Protein G |
| 克隆类型 | Polyclonal |
| 储存液 | 0.01M PBS (pH7.4). |
| 保存条件 | Store at -20℃ stable for 2 years (lyophilized powder). Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Immunoglobulin G (IgG), is one of the most abundant proteins in serum with normal levels between 8-17 mg/mL in adult blood. IgG is important for our defence against microorganisms and the molecules are produced by B lymphocytes as a part of our adaptive immune response. The IgG molecule has two separate functions; to bind to the pathogen that elicited the response and to recruit other cells and molecules to destroy the antigen. The variability of the IgG pool is generated by somatic recombination and the number of specificities in an individual at a given time point is estimated to be 1011 variants. |
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文献和实验[IF=9.9] Lijun You. et al. Magnetic polyphosphazene@Au particles as substrates for multiple-detection of immunoproteins by surface-enhanced Raman spectroscopy. J COLLOID INTERF SCI. 2023 Oct;648:1006 Other
[IF=9.518] He Q et al. Spectral-optical-tweezer-assisted fluorescence multiplexing system for QDs-encoded bead-array bioassay. Biosens Bioelectron. 2019 Mar 15;129:107-117. Other Human
[IF=8.4] Lili Zhao. et al. Target-induced photoelectrochemistry and colorimetric dual-mode platform for HIgG based on Ag2S/SnO2 composites and CoOOH nanoflakes. SENSOR ACTUAT B-CHEM. 2024 Jun;409:135638 Other
[IF=8.11] Shaoquan Zheng. et al. Landscape of cancer-associated fibroblasts identifies the secreted biglycan as a protumor and immunosuppressive factor in triple-negative breast cancer. Oncoimmunology. 2022;11(1):2020984 WB Human
[IF=7.129] Qianfeng Liu. et al. Perfluoroalkyl substances promote breast cancer progression via ERα and GPER mediated PI3K/Akt and MAPK/Erk signaling pathways. ECOTOX ENVIRON SAFE. 2023 Jun;258:114980 WB Human
E L I S A w i t h p l a t e l e t s
PBS-PF (paraformaldehyde), 2% PBS-tween 20, 0.05% glycine (x1) - BSA, 1 mg/ml revelation solution (citrate/phosphate buffer), pH 5, 1 L, water solution = citrique acid, 5.1065 g + Na2 HPO4 x 2H2 0, 9.08 g
Anti-GFP/BrdU In Situ Immunostaining
IgG (Jackson ImmunoResearch, 111-096-006) Biotinylated anti-BrdU (AbCam, ab2284-125) Streptavidin-Alexa Flour 555 (Invitrogen/Molecular Probes, S21381) Goat serum Horse serum BSA 2N HCl DAPI Fluorescent mounting medium (Vectashield
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
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