Antigen: bs-1996P, 0.2ug/100ul
Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000;
Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;
TMB(C-0024) staining;
Read the data in MicroplateReader by 450nm.
- ￥1180 - 2800
- Bioss
- bs-1996R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1996R |
| 英文名称 | CD171/L1CAM Rabbit pAb |
| 中文名称 | 神经细胞粘附分子配体1抗体 |
| 英文别名 | L1CAM_HUMAN; Neural cell adhesion molecule L1; CAML1; MIC5; N-CAM-L1; NCAM-L1; CD171; L1 cell adhesion molecule; S10; HSAS; MASA; SPG1; HSAS1; N-CAM-L1; |
| 产品应用 | Flow-Cyt=1μg /test, ELISA=1:5000-10000 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CD171 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 138 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Neurology process > Growth and Development > Axonal Guidance Proteins Neuroscience > Neurology process > Neurogenesis Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Cell Adhesion Molecules > Liver |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein. |
| 相似性 | Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family. Contains 5 fibronectin type-III domains. Contains 6 Ig-like C2-type (immunoglobulin-like) domains. |
| 功能 | Cell adhesion molecule with an important role in the development of the nervous system. Involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Binds to axonin on neurons. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | L1cam (L1 cell adhesion molecule isoform 1 precursor) is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause three X-linked neurological syndromes known by the acronym CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of a neuron-specific exon is thought to be functionally relevant. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {1μg /test} |
| {ELISA} | {1:5000-10000} |
Antigen: bs-1996P, 0.2ug/100ul
Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;
TMB(C-0024) staining;
Read the data in MicroplateReader by 450nm.
Primary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000;
Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 5000;
TMB(C-0024) staining;
Read the data in MicroplateReader by 450nm.
Blank control(blue): U937(fixed with 2% Paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-CD171 antibody(bs-1996R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA.
Isotype Control Antibody: Rabbit IgG(orange), used under the same conditions ).
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Primary Antibody:Rabbit Anti-CD171 antibody(bs-1996R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA.
Isotype Control Antibody: Rabbit IgG(orange), used under the same conditions ).
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
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文献和实验该产品被引用文献
[IF={{ 12.7 }}] {Lu Weicheng. et al. Interorgan communication in neurogenic heterotopic ossification: the role of brain-derived extracellular vesicles. BONE RES. 2024 Feb;12(1):1-16} {IHC,WB} {Rat}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
TRAF‐Mediated TNFR‐Family Signaling
interacts with the cytoplasmic domain of CD40. J. Biol. Chem. 269:30069‐30072. Hu, W.H., Mo, X.M., Walters, W.M., Brambilla, R., and Bethea, J.R. 2004. TNAP, a novel repressor
， Adu Virus Res， 55， 599， 2000. 29. Naldini， L.， BIomer， U.， Gallay， P.， Ory， D.， Mulligan， R.， Gage， F.H.， Verma， I.M.， and Trono， D.， In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector， Science， 272， 263， 1996
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CD171/L1CAM Rabbit pAb(bs-1996R)-50ul/100ul/200ul
￥1180 - 2800
