Positive control: mouse nephrocyte(2% Paraformaldehyde-fixed )
Isotype Control Antibody: Rabbit IgG;
Dilution: 1μg in 100 μl 1 X PBS containing 0.5% BSA
Secondary Antibody: Goat anti-rabbit IgG-FITC;
Dilution: 1:200 in 1 X PBS containing 0.5% BSA
Primary Antibody: rabbit Anti-GPR48 (bs-10054R);
Dilution: 1μg in 100 μl 1X PBS containing 0.5% BSA
- ￥1180 - 2800
- Bioss
- bs-10054R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10054R |
| 英文名称 | GPR48 Rabbit pAb |
| 中文名称 | G蛋白偶联受体48抗体 |
| 英文别名 | G protein coupled receptor 48; G-protein coupled receptor 48; GPR48; GPCR GPR48; Leucine-rich repeat-containing G protein-coupled receptor 4; Leucine-rich repeat-containing G-protein coupled receptor 4; LGR4; LGR4_HUMAN. |
| 产品应用 | Flow-Cyt=2μg/Test Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human LGR4 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 105 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Signaling Pathway > G Protein Signaling > GPCR |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
| 组织特异性 | Expressed in multiple steroidogenic tissues: placenta, ovary, testis and adrenal. Expressed also in spinal cord, thyroid, stomach, trachea, heart, pancreas, kidney, prostate and spleen. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. Contains 15 LRR (leucine-rich) repeats. Contains 1 LRRNT domain. |
| 功能 | Orphan receptor. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | G protein-coupled receptors (GPCRs), also designated seven transmembrane (7TM) receptors or heptahelical receptors, interact with G proteins (heterotrimeric GTPases) to synthesize intracellular second messengers, such as diacylglycerol, cyclic AMP, inositol phosphates and calcium ions. Their diverse biological functions range from vision and olfaction to neuronal and endocrine signaling and are involved in many pathological conditions. LGR4 (leucine-rich repeat-containing G protein-coupled receptor 4), also known as GPR48, is a 951 amino acid multi-pass membrane protein that contains 15 LRR (leucine-rich repeats) and belongs to the GPCR family. Expressed in multiple tissues, including testis, ovary, placenta, stomach, heart, kidney, pancreas and spleen, LGR4 functions as an orphan receptor that may be involved in physiologic activities throughout the cell. LGR4 is overexpressed in various cancer types and is thought to enhance carcinoma invasiveness and metastasis, suggesting an important role in tumor progression. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {2μg/Test} |
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于100mL亚硒酸盐肮氨酸（SC）增菌液内，于36±1℃培养18~24h。5.2 分离取增菌液1环，划线接种于一个亚硫酸铋琼脂平板和一个DHL琼脂平板。两种增菌液可同时划线接种在同一个平板上。于36±1℃分别培养18~24h（DHL）或40~48h（BS），观察各个平板上生长的菌落，沙门氏菌Ⅰ、Ⅱ、Ⅳ、Ⅴ、Ⅵ和沙门氏菌Ⅲ在各个平板上的菌落特征见表1。表1 沙门氏菌属各群在各种选择性琼脂平板上的菌落特征5.3 生化试验 5.3.1 自选择性琼脂平板上直接挑取数个可疑菌落，分别接种三糖铁琼脂。在三糖
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GPR48 Rabbit pAb(bs-10054R)-50ul/100ul/200ul
￥1180 - 2800
