IGF2 Recombinant Rabbit mAb(bs

m-52776R)-50ul/100ul

价格￥1400 - 2500

品牌Bioss
货号bsm-52776R
更新日期2025年10月24日

应用范围产品信息以Bioss网站为准

10 年钻石会员

相关产品推荐更多 >

万千商家帮你免费找货

0 人在求购买到急需产品

详细信息
文献和实验
技术资料

应用范围：
产品信息以Bioss网站为准
规格：
50ul/100ul

规格：	50ul	产品价格：	￥1400.0
规格：	100ul	产品价格：	￥2500.0

产品编号	bsm-52776R
英文名称	IGF2 Recombinant Rabbit mAb
中文名称	胰岛素样生长因子-II重组兔单抗
英文别名	IGF2_HUMAN; Insulin-like growth factor II; IGF-II; IGF II; Somatomedin-A; T3M-11-derived growth factor; Insulin-like growth factor II; Insulin-like growth factor II Ala-25 Del; Preptin; PP1446; Insulin like Growth Factor 2;
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:400-800, IF=1:100-500 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Human
抗体来源	Rabbit
免疫原	Recombinant human IGF2 protein
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Recombinant
理论分子量	11 kDa
浓度	1mg/ml
储存液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域	Cancer > Growth factors > Insulin and insulin-like Developmental Biology > Post embryonic development > Growth hormones Kits/ Lysates/ Other > Kits > ELISA Kits > ELISA Kits > Growth factors and hormones ELISA kits Signal Transduction > Growth Factors/Hormones > Insulin / Insulin-like
亚细胞定位	Secreted.
翻译后修饰	O-glycosylated with core 1 or possibly core 8 glycans. Thr-96 is a minor glycosylation site compared to Thr-99.
相似性	Belongs to the insulin family.
功能	The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	The IGF2 gene encodes a member of the insulin family of polypeptide growth factors that is involved in development and growth. It is expressed only from the paternally inherited allele and is an imprinted gene which is a candidate gene for eating disorders. There is a read-through, INS-IGF2, which aligns to this gene at the 3' region and to the upstream INS gene at the 5' region. Two alternatively spliced transcript variants encoding the same protein have been found for this gene. Insulin Like Growth Factor-2 (IGF-2) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types.

应用	推荐稀释比例
{WB}	{1:500-2000}
{IHC-P}	{1:100-500}
{IHC-F}	{1:400-800}
{IF}	{1:100-500}

Western blot analysis of IGF2 on human placenta tissue lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (bsm-52776R, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.

Predicted band size: 20 kDa
Observed band size: 11 kDa

Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-IGF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (bsm-52776R, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-IGF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (bsm-52776R, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-IGF2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes.The tissues were blocked in 1% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (bsm-52776R, 1/50) for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

P‌‌araformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IGF2) Monoclonal Antibody, Unconjugated (bsm-52776R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

文献和实验

大家都在搜

大家都在搜