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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-15598R |
| 英文名称 | Elastase-2B Rabbit pAb |
| 中文名称 | 胰腺弹性蛋白酶2B抗体 |
| 英文别名 | CEL2B_HUMAN; Chymotrypsin-like elastase family member 2B; Elastase-2B. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Elastase-2B |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 26 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cell Biology > Proteolysis / Ubiquitin > Proteolytic enzymes Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Proteins > Collagen Signal Transduction > Cytoskeleton / ECM > Extracellular Matrix > ECM Proteins > Elastin |
| 亚细胞定位 | Secreted._x000D_ |
| 组织特异性 | Pancreas._x000D_ |
| 相似性 | Belongs to the peptidase S1 family. Elastase subfamily. Contains 1 peptidase S1 domain. |
| 功能 | Acts upon elastin._x000D_ |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have six elastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike other elastases, elastase 3A has little elastolytic activity. Like most of the human elastases, elastase 3A is secreted from the pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has a digestive function in the intestine. Elastase 3A preferentially cleaves proteins after alanine residues. Elastase 3A may also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B have been referred to as protease E and as elastase 1. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验Testing Rabbit Bleeds By Elisa
same amount of NaBH4 after 15''. Do a quick microfuge spin if there are too many bubbles. B. ELISA with Peptide conjugated to BSA Coat 10 ug/ml antigen (peptide conjugated to BSA) diluted in TBS (50 ul/well) ON at 4 deg C. Remove antigen
Polyclonal Antibody Production 简单快捷制备多抗方法，可用于western、ELISA和免疫/b
Very useful for rapid and simple generation of antibodies for western blots, ELISA assays, and immunoprecipitation. -------------------------------------------------------------------------------- Rabbit
Testing Rabbit Bleeds By Elisa
Testing Rabbit Bleeds By Elisa Claire Walczak January 1996 You can test whether or not you have gotten an immune response to the peptide and how strong that immune response is by doing ELISAs against peptide conjugated to BSA
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