Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-GOT2 (bs-10557R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
- ￥1180 - 2800
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- bs-10557R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-10557R |
| 英文名称 | GOT2 Rabbit pAb |
| 中文名称 | 谷草转氨酶2抗体 |
| 英文别名 | ASPATA; FABP pm; Got 2; Aspartate aminotransferase; Aspartate aminotransferase 2; Aspartate aminotransferase, mitochondrial; EC 2.6.1.1; FABP 1; FABPpm; Fatty acid binding protein; FLJ40994; Glutamate oxaloacetate transaminase 2; Glutamate oxaloacetate transaminase 2, mitochondrial; Glutamate oxaloacetate transaminase, mitochondrial; Glutamic oxaloacetic transaminase 2, mitochondrial(aspartate aminotransferase 2); KAT4; KATIV; Kynurenine aminotransferase IV; mAspAT; mitAAT; Mitochondrial aspartate aminotransferase; OTTMUSP00000017748; Plasma membrane fatty acid binding protein; Plasma membrane-associated fatty acid-binding protein; Transaminase A; MGC102129; MGC115763; AL022787; AATM_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GOT2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 45 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Cancer Metabolism > Metabolic signaling pathway > Metabolism of carbohydrates Metabolism > Pathways and Processes > Metabolic signaling pathways > Amino acid metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Carbohydrate metabolism Signal Transduction > Metabolism > Amino Acids |
| 亚基 | Homodimer. |
| 亚细胞定位 | Mitochondrion matrix. Cell membrane. Note=Exposure to alcohol promotes translocation to the cell membrane. |
| 相似性 | Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. |
| 功能 | Catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid (KA). Plays a key role in amino acid metabolism. Important for metabolite exchange between mitochondria and cytosol. Facilitates cellular uptake of long-chain free fatty acids. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | GOT2 [Glutamate oxaloacetate transaminase] is a ubiquitous pyridoxal phosphate dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene. Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
Muscle(Mouse) Lysate at 40 ug
Primary: Anti-GOT2 (bs-10557R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
Muscle(Mouse) Lysate at 40 ug
Primary: Anti-GOT2 (bs-10557R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 45 kD
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文献和实验相关实验
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GOT2 Rabbit pAb(bs-10557R)-50ul/100ul/200ul
￥1180 - 2800
