Sample: Liver (Mouse) Lysate at 40 ug
Primary: Anti- PXR (bs-2334R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
- ￥1180 - 2800
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- bs-2334R
- 2025年10月24日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2334R |
| 英文名称 | PXR Rabbit pAb |
| 中文名称 | 孤儿核受体PAR1抗体 |
| 英文别名 | BXR; NR1I2; Nuclear receptor subfamily 1 group I member 2; ONR 1; ONR1; Orphan nuclear receptor PAR 1; Orphan nuclear receptor PAR1; Orphan nuclear receptor PXR; PAR 1; PAR 2; PAR; PAR q; PAR1; PAR2; PARq; Pregnane X receptor; PRR; SAR; Steroid and xenobiotic receptor; SXR. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human PXR |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 48 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Orphan Nuclear Receptors Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Metabolism > Pathways and Processes > Metabolic signaling pathways > Drug metabolism Metabolism > Types of disease > Cancer Signal Transduction > Growth Factors/Hormones > Hormones Signal Transduction > Metabolism > Drug metabolism |
| 亚基 | Heterodimer with RXR. Interacts with NCOA1. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | Expressed in liver, colon and small intestine. |
| 相似性 | Belongs to the nuclear hormone receptor family. NR1 subfamily. Contains 1 nuclear receptor DNA-binding domain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The human nuclear pregnane X receptor (PXR) activates cytochrome P450-3A expression in response to a wide variety of xenobiotics and plays a critical role in mediating dangerous drug-drug interactions. The human PXR is related to the mouse Pxr1, which they had cloned and shown to be activated by dexamethasone, pregnenolone 16-alpha-carbonitrile (PCN), and other compounds known to induce expression of the CYP3A1 gene, the predominant form of CYP3A in rat liver and intestine. Northern blot analysis detected most abundant expression in liver, colon, and small intestine; transcripts of 2.6, 4.3, and 5 kb were present in each of these tissues. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {2ug/Test} |
Blank control:HepG2.
Primary Antibody (green line): Rabbit Anti-PXR antibody (bs-2334R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
Primary Antibody (green line): Rabbit Anti-PXR antibody (bs-2334R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验该产品被引用文献
[IF={{ 5.58 }}] {Zhou, Xiaoqiao, et al. "Cecropin B Represses CYP3A29 Expression through Activation of the TLR2/4-NF-κB/PXR Signaling Pathway." Scientific Reports6 (2016): 27876.} {Other} {="Pig"}
[IF={{ 5.014 }}] {Desirée Bartolini. et al. Alpha-Tocopherol Metabolites (The Vitamin E Metabolome) and Their Interindividual Variability during Supplementation. Antioxidants-Basel. 2021 Feb;10(2):173} {WB} {Human}
[IF={{ 4.9 }}] {Takanori Matsui. et al.Apixaban Inhibits Progression of Experimental Diabetic Nephropathy by Blocking Advanced Glycation End Product-Receptor Axis.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES.} {IHC} {}
[IF={{ 4.757 }}] {Xianyu Huang. et al. Transcriptome Analysis of Protection by Dendrobium Nobile Alkaloids (DNLA) against Chronic Alcoholic Liver Injury in Mice. BIOMEDICINES. 2022 Nov;10(11):2800} {WB} {Mouse}
[IF={{ 4.4 }}] {Shantong Qiu. et al. HNF4α improves hepatocyte regeneration by upregulating PXR. FASEB J. 2024 Jul;38(14):e23830} {IF,WB} {Mouse}
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PXR Rabbit pAb(bs-2334R)-50ul/100ul/200ul
￥1180 - 2800
