Blank control:HepG2.
Primary Antibody (green line): Rabbit Anti-Phospho-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) antibody (bs-3137R)
Dilution: 2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
- Bioss
- bs-3137R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-3137R |
| 英文名称 | phospho-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) Rabbit pAb |
| 中文名称 | 磷酸化叉头蛋白家族抗体 |
| 英文别名 | FOXO4(phospho T32); p-FoxO1(Thr24)/FoxO3a(Thr32); FOXO3A(phospho T32); p-FOXO3A(phospho T32); Forkhead box protein O1; Afxh; AI876417; FKHR; Fkhr1; Foxo1a; Forkhead; FKH 1; FKH1; FKH1; FKHR; FKHR; Forkhead(Drosophila) homolog 1(rhabdomyosarcoma); Forkhead(Drosophila) homolog 1(rhabdomyosarcoma); Forkhead box O1; Forkhead box protein O1; Forkhead box protein O1A; Forkhead in rhabdomyosarcoma; Forkhead, Drosophila, homolog of, in rhabdomyosarcoma; FOXO1; FOXO1; FOXO1_HUMAN; FOXO1A; forkhead box protein O3. |
| 产品应用 | Flow-Cyt=2ug/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Dog, Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated Synthesised phosphopeptide derived from human FOXO1 around the phosphorylation site of Thr24 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 74 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Signal transduction > Nuclear signaling > Nuclear hormone receptors > Coactivators and repressors Epigenetics and Nuclear Signaling > Nuclear Signaling Pathways > Nuclear Receptors > Co-activators/co-repressors Epigenetics and Nuclear Signaling > Transcription > Domain Families > Forkhead Box > FOXO Metabolism > Types of disease > Obesity Neuroscience > Neurology process > Neurogenesis Signal Transduction > Signaling Pathway > Nuclear Signaling > Nuclear Hormone Receptors > Co-activators/co-repressors |
| 亚基 | Interacts with LRPPRC. Interacts with SIRT1 and this interaction requires the presence of KRIT1. Interacts with NLK. Binds to CDK1 and 14-3-3 proteins. |
| 亚细胞定位 | Cytoplasm. Nucleus. Note=Shuttles between cytoplasm and nucleus. Translocates to the nucleus upon oxidative stress induced phosphorylation at Ser-212 by STK4/MST1. Translocates to the nucleus upon phosphorylation of Thr-24, Ser-256 and Ser-322 by SGK1. |
| 组织特异性 | Ubiquitous. |
| 翻译后修饰 | Phosphorylated by AKT1; insulin-induced. Phosphorylated by NLK, which inhibits transcriptional activity and promotes nuclear export (By similarity). IGF1 rapidly induces phosphorylation of Ser-256, Thr-24, and Ser-319. Phosphorylation of Ser-256 decreases DNA-binding activity and promotes the phosphorylation of Thr-24, and Ser-319, permitting phosphorylation of Ser-322 and Ser-325, probably by CK1, leading to nuclear exclusion and loss of function. Phosphorylation of Ser-329 is independent of IGF1 and leads to reduced function. Phosphorylated upon DNA damage, probably by ATM or ATR. Phosphorylation of Ser-249 by CDK1 disrupts 14-3-3 proteins binding and thereby promotes FOXO1 nuclear accumulation and subsequent transcription activation and cell death. Phosphorylated by STK4/MST1 on Ser-212 upon oxidative stress. Phosphorylated on Thr-24, Ser-256 and Ser-322 by SGK1 resulting in its translocation from the nucleus to the cytoplasm. |
| 相似性 | Contains 1 fork-head DNA-binding domain. |
| 功能 | Transcription factor which acts as a regulator of cell responses to oxidative stress. In the presence of KIRT1, mediates down-regulation of cyclin D1 and up-regulation of CDKN1B levels which are required for cell transition from proliferative growth to quiescence. Triggers death of postmitotic neurons when phosphorylated by CDK1. Activates transcription of PMAIP1. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | This gene belongs to the forkhead family of transcription factors which are characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in myogenic growth and differentiation. Translocation of this gene with PAX3 has been associated with alveolar rhabdomyosarcoma. [provided by RefSeq]. |
| 应用 | 推荐稀释比例 |
| {Flow-Cyt} | {2ug/Test} |
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phospho-FOXO1 + FOXO3 + FOXO4 (phospho T24 + T32) Rabbit pAb(bs-3137R)-50ul/100ul/200ul
￥1180 - 2800
