Tissue/cell: human lung carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_
Incubation: Anti-HLA B/HLA G Polyclonal Antibody, Unconjugated(bs-0752R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_
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50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-0752R |
| 英文名称 | HLA G Rabbit pAb |
| 中文名称 | 人类白细胞抗原G抗体 |
| 英文别名 | B2 microglobulin; DADB-15K14.8; HLA 6.0; HLA 60; HLA class I histocompatibility antigen alpha chain G; HLA class I histocompatibility antigen alpha chain G precursor; HLA class I histocompatibility antigen, alpha chain G; HLA class I molecule; HLA G; HLA G antigen; HLA G histocompatibility antigen class I G; HLA G protein; HLA G2.2; HLA G3; HLA-G; HLA-G histocompatibility antigen, class I; HLA60; HLAG; HLAG_HUMAN; Human Leukocyte Antigen G Histocompatability Antigen Class 1; Lymphocyte antigen; Major histocompatibility complex class I G; MHC class I antigen; MHC class I antigen G; MHC class Ib antigen; MHC G; Murine homolog of Qa; T Cell A Locus; T-cell A locus; TCA. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from the middle of human HLA-G |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 35 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Adaptive Immunity > MHC > Class I Immunology > Immune System Diseases > Allergens |
| 亚基 | Heterodimer of an alpha chain and a beta chain (beta-2-microglobulin). Homodimer; disulfide-linked. Binds to LILRB1 and LILRB2. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Expressed in trophoblasts. |
| 相似性 | Belongs to the MHC class I family._x000D_
Contains 1 Ig-like C1-type (immunoglobulin-like) |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | HLA-G belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-G is expressed on fetal derived placental cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domain, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exon 6 encodes the cytoplasmic tail. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |


Sample:
_x000D_ Brain (Rat) Lysate at 30 ug
_x000D_ Kidney (Rat) lysate at 30 ug
_x000D_ Primary: Anti- HLA G (bs-0752R) at 1/200 dilution
_x000D_ Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution
_x000D_ Predicted band size: 35 kD
_x000D_ Observed band size: 35 kD
_x000D_
_x000D_ Brain (Rat) Lysate at 30 ug
_x000D_ Kidney (Rat) lysate at 30 ug
_x000D_ Primary: Anti- HLA G (bs-0752R) at 1/200 dilution
_x000D_ Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/3000 dilution
_x000D_ Predicted band size: 35 kD
_x000D_ Observed band size: 35 kD
_x000D_
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MHC I | HLA-G Rabbit pAb(bs-0752R)-50ul/100ul/200ul
¥1180 - 2800










