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KDM1/LSD1 Rabbit pAb(bs-10166R

)-50ul/100ul/200ul
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  • ￥1180 - 2800
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  • bs-10166R
  • 2025年10月16日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-10166R
    英文名称KDM1/LSD1 Rabbit pAb
    中文名称组蛋白赖氨酸特异性脱甲基酶1抗体
    英文别名Amine oxidase(flavin containing) domain 2; Amine oxidase flavin containing domain 2; Amine oxidase flavin containing domain protein 2; AOF 2; AOF2; BHC110; BRAF35 HDAC complex protein BHC110; BRAF35-HDAC complex protein BHC110; FAD binding protein BRAF35 HDAC complex, 110 kDa subunit; Flavin containing amine oxidase domain containing protein 2; Flavin-containing amine oxidase domain-containing protein 2; KDM 1; Kdm1a; KDM1A_HUMAN; KIAA0601; LSD 1; LSD1; Lysine(K) specific demethylase 1; Lysine(K) specific demethylase 1A; Lysine specific histone demethylase 1; Lysine specific histone demethylase 1A; Lysine-specific histone demethylase 1; Lysine-specific histone demethylase 1A.
    产品应用WB=1:500-2000, ICC/IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Rabbit)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human KDM1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量94 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > Chromatin Modifying Enzymes > Methylation > Lysine demethylation

    亚基Component of a RCOR/GFI/KDM1A/HDAC complex. Interacts directly with GFI1 and GFI1B (By similarity). Component of a BHC histone deacetylase complex that contains HDAC1, HDAC2, HMG20B, KDM1A, RCOR1 and PHF21A. The BHC complex may also contain ZMYM2, ZNF217, ZMYM3, GSE1 and GTF2I. In the complex, RCOR1/CoREST strongly enhances the demethylase activity and protects it from the proteasome while PHF21A/BHC80 inhibits the demethylase activity. Interacts with the androgen receptor (AR). Interacts with ASXL1.
    亚细胞定位Nucleus.
    组织特异性Ubiquitously expressed.
    相似性Belongs to the flavin monoamine oxidase family.
    Contains 1 SWIRM domain.
    功能Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Gene activation and repression is specifically regulated by histone methylation status at distinct lysine residues. Lysine specific demethylase 1 (KDM1/LSD1) is a long-sought histone demethylase that specifically demethylates mono and di methyl histone H3 at K4 and K9. Thus KDM1 is a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {ICC/IF}{1:100-500}

     

    产品细节图片1
    Hela cell; 4% P‌‌araformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (KDM1/LSD1) polyclonal Antibody, Unconjugated (bs-10166R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
    产品细节图片2
    Sample: placenta (Mouse) Lysate at 40 ug
    Primary: Anti-KDM1(bs-10166R) at 1/300 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 94 kD
    Observed band size: 94 kD
    产品细节图片3
    Sample:
    Lane 1: Testis (Mouse) Lysate at 40 ug
    Lane 2: Uterus (Mouse) Lysate at 40 ug
    Lane 3: NIH/3T3 (Mouse) Cell Lysate at 30 ug
    Lane 4: Testis (Rat) Lysate at 40 ug
    Lane 5: Hela (Human) Cell Lysate at 30 ug
    Lane 6: 293T (Human) Cell Lysate at 30 ug
    Lane 7: Jurkat (Human) Cell Lysate at 30 ug
    Primary: Anti-KDM1/LSD1 (bs-10166R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 120 kD
    Observed band size: 120 kD

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