Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37鈩?? for 20 min;
Incubation: Anti-MSX1 Polyclonal Antibody, Unconjugated(bs-8512R) 1:200, overnight at 4掳C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-8512R
- 2025年10月16日
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-8512R |
| 英文名称 | MSX1 Rabbit pAb |
| 中文名称 | MSH同源蛋白1样蛋白抗体 |
| 英文别名 | Msh homeobox 1 like protein; Homeobox 7; Homeobox protein Hox-7; Homeobox protein MSX 1; Homeobox protein MSX-1; Homeobox protein MSX1; Homeobox, msh like 1; Homeobox, msh-like 1; HOX 7; Hox 7.1; Hox-7; HOX7; Hox7.1; HYD 1; HYD1; msh(Drosophila) homeo box homolog 1(formerly homeo box 7); Msh; msh homeo box 1; msh homeo box homolog 1; Msh homeobox 1; Msh homeobox 1-like protein; msh homeobox homolog 1(Drosophila); msh homeobox homolog 1; MSH, Drosophila, Homolog of, 1; MSX 1; MSX1; MSX1_HUMAN; Muscle segment homeobox; Muscle segment homeobox, Drosophila, Homolog of, 1; OFC5; STHAG1. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=5μg /Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Cow) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MSX1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 31 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Organogenesis > Nervous system development Developmental Biology > Organogenesis > Skeletal development > Bone Epigenetics and Nuclear Signaling > Transcription > Domain Families > Developmental Families > HOX Epigenetics and Nuclear Signaling > Transcription > Transcription Factors Neuroscience > Cell Type Marker > Neural Stem Cell marker Neuroscience > Neurology process > Neurogenesis Stem Cells > Neural Stem Cells > Intracellular |
| 亚细胞定位 | Nucleus |
| 组织特异性 | Expressed in the developing nail bed mesenchyme. |
| 翻译后修饰 | Sumoylated by PIAS1, desumoylated by SENP1 (By similarity). |
| 相似性 | Belongs to the Msh homeobox family. Contains 1 homeobox DNA-binding domain. |
| 功能 | Acts as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis. Expression in the developing nail bed mesenchyme is important for nail plate thickness and integrity. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Acts as a transcriptional repressor. May play a role in limb-pattern formation. Acts in cranofacial development and specifically in odontogenesis. Expression in the developing nail bed mesenchyme is important for nail plate thickness and integrity. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {5μg /Test} |
Positive control: Hela cells
Concebtration: 5μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.
Concebtration: 5μg/10^6 cells
Incubation conditions: Avoid light , 30 minutes on the ice.
Sample:
Utreus (Mouse) Lysate at 40 ug
Hela Cell (Human) Lysate at 40 ug
Siha Cell (Human) Lysate at 40 ug
A673 Cell (Human) Lysate at 40 ug
Primary: Anti- MSX1 (bs-8512R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Utreus (Mouse) Lysate at 40 ug
Hela Cell (Human) Lysate at 40 ug
Siha Cell (Human) Lysate at 40 ug
A673 Cell (Human) Lysate at 40 ug
Primary: Anti- MSX1 (bs-8512R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
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文献和实验该产品被引用文献
[IF={{ 8 }}] {Si-Ting Chen. et al. The activation of cGAS-STING pathway causes abnormal uterine receptivity in aged mice. AGING CELL. 2024 Aug;:e14303} {IF} {Mouse}
[IF={{ 4.9 }}] {Hong-Yuan Yang. et al. Effects of Acrylamide on Mouse Implantation and Decidualization. INT J MOL SCI. 2025 Jan;26(9):4129} {IF} {Mouse}
[IF={{ 3.6 }}] {Chen Bangjie. et al. An angiogenesis-associated gene-based signature predicting prognosis and immunotherapy efficacy of head and neck squamous cell carcinoma patients. J CANCER RES CLIN. 2024 Feb;150(2):1-23} {IHC,WB} {Human}
[IF={{ 3.337 }}] {Linlin Yang. et al. Identification and Validation of MSX1 as a Key Candidate for Progestin Resistance in Endometrial Cancer. Oncotargets Ther. 2020; 13: 11669–11688} {WB} {Human}
[IF={{ 3 }}] {Qingbo Zheng. et al. Interpretation of the Yak Skin Single-Cell Transcriptome Landscape. ANIMALS. 2023 Jan;13(24):3818} {IF} {Bovine}
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MSX1 Rabbit pAb(bs-8512R)-50ul/100ul/200ul
￥1180 - 2800
