Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
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Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
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Incubation: Anti-LRIG1 Polyclonal Antibody, Unconjugated(bs-1844R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- ￥1180 - 2800
- Bioss
- bs-1844R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1844R |
| 英文名称 | LRIG1 Rabbit pAb |
| 中文名称 | LRIG1抗体 |
| 英文别名 | leucine-rich repeat protein LRIG1; leucine rich repeats and immunoglobulin-like domains 1; Leucine rich repeat protein; leucine rich repeats and immunoglobulin like domains 1; LIG 1; LIG-1; LRIG1_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human LRIG1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 120 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cancer > Oncoproteins/suppressors > Tumor suppressors |
| 亚基 | Interacts with EGFR/ERBB1, ERBB2, ERBB3 and ERBB4. |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Widely expressed. |
| 相似性 | Contains 3 Ig-like C2-type (immunoglobulin-like) domains.Contains 15 LRR (leucine-rich) repeats. Contains 1 LRRCT domain. Contains 1 LRRNT domain. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | LRIG1 protein is coded by a gene on a chromosome band 3p14.3, a region known to be deleted in various human cancers. It is considered to be a tumour suppressor gene. LRIG1 is an integral cell-surface membrane protein that is expressed by specific cells in various human tissues. Its 143kDa form may be cleaved into 111kDa and 32kDa fragments. The protein may inhibit the growth of tumors of glial cells and the down-regulation of the LRIG1 gene may be involved in the development and progression of the tumor. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验该产品被引用文献
[IF=2.272] Yang et al. Lrig1 is a positive prognostic marker in hepatocellular carcinoma. (2016) Onco.Targets.The. 9:7071-7079 WB Human
[IF=0] Marlene Happe. et al. LRIG1-3 in gliomas: LRIG1 protein expression decreased in higher grade gliomas. ONCOTARGET. 2025 Nov;16:793 WB Human
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LRIG1 | LIG-1 Rabbit pAb(bs-1844R)-50ul/100ul/200ul
￥1180 - 2800
