Blank control: Molt-4 Cells(blue).
Primary Antibody: Rabbit Anti-CD166/FITC Conjugated antibody (bs-1251R/AF488), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG/AF488orange) ,used under the same conditions.
Protocol
The cells were fixed with 2% Paraformaldehyde (10 min) . The cells were washed twice with 1 X PBS. The cells were incubated in 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions followed by the incubated with antibody (bs-1251R/AF488, 1μg /1x10^6 cells) for 30 min on ice. Acquisition of 20,000 events was performed.
- ￥1180 - 2800
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- bs-1251R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1251R |
| 英文名称 | CD166 Rabbit pAb |
| 中文名称 | 活化白细胞粘附分子抗体 |
| 英文别名 | activated leukocyte cell adhesion molecule; ALCAM protein; ALCAM; CD 166; CD166 antigen; FLJ38514; MEMD; MGC71733; FLJ3851; AI853494; BEN; DM-GRASP; MGC27910; MuSC; SC1; CD166_HUMAN. |
| 产品应用 | WB=1:500-2000, Flow-Cyt=1μg/Test Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CD166 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 62 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Immunology > Adaptive Immunity > B Cells > CD Immunology > Adaptive Immunity > T Cells > CD Kits/ Lysates/ Other > Kits > ELISA Kits > ELISA Kits > CD markers ELISA kits |
| 亚细胞定位 | Membrane; Single-pass type I membrane protein. |
| 组织特异性 | Spleen, placenta, liver, and weakly in liver. Expressed by activated T-cells, B-cells, monocytes and thymic epithelial cells. Expressed by neurons in the brain. Restricted expression in tumor cell lines. Preferentially expressed in highly metastasizing melanoma cell lines. |
| 相似性 | Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 2 Ig-like V-type (immunoglobulin-like) domains. |
| 功能 | Cell adhesion molecule that binds to CD6. Involved in neurite extension by neurons via heterophilic and hemophilic interactions. May play a role in the binding of T- and B-cells to activated leukocytes, as well as in interactions between cells of the nervous system. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | CD166 is a member of the Ig superfamily and is expressed on activated T-cells, B cells and other cells including thymic epithelial cells, fibroblasts, keratinocytes and neurons. CD6 has been identified as a receptor for CD166.The expression of CD166 is up-regulated in low-grade prostate tumors and down-regulated in high-grade tumors; may play role in progression of prostate cancer. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {Flow-Cyt} | {1μg/Test} |
Sample:
MCF-7 Cell (Human) Lysate at 30 ug
Primary: Anti-CD166 (bs-1251R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62/70 kD
MCF-7 Cell (Human) Lysate at 30 ug
Primary: Anti-CD166 (bs-1251R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62/70 kD
Sample:
HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-CD166 (bs-1251R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62/70 kD
HepG2 Cell (Human) Lysate at 30 ug
Primary: Anti-CD166 (bs-1251R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62 kD
Observed band size: 62/70 kD
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文献和实验该产品被引用文献
[IF={{ 6.081 }}] {Qianyu Cheng. et al. Establishing and characterizing human stem cells from the apical papilla immortalized by hTERT gene transfer. FRONT CELL DEV BIOL. 2023; 11: 1158936} {ICC} {Human}
[IF={{ 4.486 }}] {Wenping Luo. et al. BMP9‐initiated osteogenic/odontogenic differentiation of mouse tooth germ mesenchymal cells (TGMCS) requires Wnt/β‐catenin signalling activity. 2021 Feb 18} {IF} {Mouse}
[IF={{ 4.175 }}] {Yue Wu. et al. LncRNA WDFY3-AS2 promotes cisplatin resistance and the cancer stem cell in ovarian cancer by regulating hsa-miR-139-5p/SDC4 axis. Cancer Cell Int. 2021 Dec;21(1):1-14} {FCM} {Human}
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[IF={{ 3.739 }}] {Vašíček J et al. Combined approach for characterization and quality assessment of rabbit bone marrow-derived mesenchymal stem cells intended for gene banking. N Biotechnol. 2019 Aug 7;54:1-12.} {FCM} {Rabbit}
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CD166 Rabbit pAb(bs-1251R)-50ul/100ul/200ul
￥1180 - 2800
