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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1159R |
| 英文名称 | P-cadherin Rabbit pAb |
| 中文名称 | P-钙粘附分子抗体 |
| 英文别名 | placental; Cadherin-3; P cadherin; Cadp; Placental cadherin; P-cadherin; AI385538; Pca; Cadherin 3 precursor; Cadherin 3 type 1; Calcium dependent adhesion protein placental; Cadherin; CDH3; CDHP; 7B4 antigen; Cadherin-1; Cadherin-2; Cadherin-4; Cadherin-5; CAM 120/80; CD144; CD324; CD325; Cdh4; CDH5; CDHE; CDHN; CADH3_HUMAN; E-cadherin; Epithelial cadherin; N-cadherin; NCAD; Neural cadherin; P-cadherin; UVO; Uvomorulin; Vascular endothelial cadherin; CDH 3; CDH3; CDH3 protein; CDHP; HJMD; PCAD; Placental cadherin. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Chicken, Dog, Cow, Rabbit, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human P-cadherin |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 80 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Sensory System > Visual system Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Cadherins |
| 亚基 | Interacts with CDCP1. |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein. |
| 组织特异性 | Expressed in some normal epithelial tissues and in some carcinoma cell lines. |
| 相似性 | Contains 5 cadherin domains. |
| 功能 | Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | cell-cell adhesion molecules. Cadherins are responsible for a whole range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. N-cadherin is found in many locations including cardiac adherins junctions, oral squamous epithelial cells, and breast epithelial cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {2μg/Test} |
_x000D_ Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-P-cadherin Polyclonal Antibody, Unconjugated(bs-1159R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
_x000D_
_x000D_ MCF-7(Human) Cell Lysate at 30 ug
_x000D_ Primary: Anti-P-cadherin (bs-1159R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 80 kD
_x000D_ Observed band size: 90 kD
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文献和实验[IF=5.232] Chen F et al. Integrated Analysis of Quantitative Proteome and Transcriptional Profiles Reveals the Dynamic Function of Maternally Expressed Proteins After Parthenogenetic Activation of Buffalo Oocyte.Mol Cell Proteomics. 2018 Oct;17(10):1875-1891. WB Buffalo
[IF=2] Li Changcheng. et al. MicroRNA Expression Profiling and Functional Analysis of CDH3 during Oogenesis in the Chinese Alligator (Alligator sinensis). Current Zoology. 2025 Aug;: IF,WB Chinese alligator
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T 1000 进口分装 F0031 降钙素(Calcitonin) ELISA 48T/96T 1500/2500 进口分装 F0032 P- 钙粘着蛋白（ P-Cadherin) ELISA 48T/96T 2000/3000 进口分装 F0033 环磷酸腺苷(cAMP) ELISA 48T/96T 1500/2500 进口分装 F0034 心营养素-1(Cardiotrophin-1) ELISA 48T/96T 2000/3000
T 1000 进口分装 F0031 降钙素(Calcitonin) ELISA 48T/96T 1500/2500 进口分装 F0032 P- 钙粘着蛋白（ P-Cadherin) ELISA 48T/96T 2000/3000 进口分装 F0033 环磷酸腺苷(cAMP) ELISA 48T/96T 1500/2500 进口分装 F0034 心营养素-1(Cardiotrophin-1) ELISA 48T/96T 2000/3000
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