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- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1159R |
| 英文名称 | P-cadherin Rabbit pAb |
| 中文名称 | P-钙粘附分子抗体 |
| 英文别名 | placental; Cadherin-3; P cadherin; Cadp; Placental cadherin; P-cadherin; AI385538; Pca; Cadherin 3 precursor; Cadherin 3 type 1; Calcium dependent adhesion protein placental; Cadherin; CDH3; CDHP; 7B4 antigen; Cadherin-1; Cadherin-2; Cadherin-4; Cadherin-5; CAM 120/80; CD144; CD324; CD325; Cdh4; CDH5; CDHE; CDHN; CADH3_HUMAN; E-cadherin; Epithelial cadherin; N-cadherin; NCAD; Neural cadherin; P-cadherin; UVO; Uvomorulin; Vascular endothelial cadherin; CDH 3; CDH3; CDH3 protein; CDHP; HJMD; PCAD; Placental cadherin. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2μg/Test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Chicken, Dog, Cow, Rabbit, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human P-cadherin |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 80 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Sensory System > Visual system Signal Transduction > Cytoskeleton / ECM > Cell Adhesion > Cadherins |
| 亚基 | Interacts with CDCP1. |
| 亚细胞定位 | Cell membrane; Single-pass type I membrane protein. |
| 组织特异性 | Expressed in some normal epithelial tissues and in some carcinoma cell lines. |
| 相似性 | Contains 5 cadherin domains. |
| 功能 | Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | cell-cell adhesion molecules. Cadherins are responsible for a whole range of processes including development, wound healing, cell-cell signaling, cell growth and differentiation. N-cadherin is found in many locations including cardiac adherins junctions, oral squamous epithelial cells, and breast epithelial cells. Studies have linked N-cadherin to cancer metastasis by showing the aggressive tumor cells had preferentially turned on N-cadherin as opposed to E- or P-cadherin. Cadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {2μg/Test} |
Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-P-cadherin Polyclonal Antibody, Unconjugated(bs-1159R) 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated(bs-0295G-Cy3)used at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue,C-0033) was used to stain the cell nuclei
MCF-7(Human) Cell Lysate at 30 ug
Primary: Anti-P-cadherin (bs-1159R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 90 kD
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文献和实验[IF={{ 5.232 }}] {Chen F et al. Integrated Analysis of Quantitative Proteome and Transcriptional Profiles Reveals the Dynamic Function of Maternally Expressed Proteins After Parthenogenetic Activation of Buffalo Oocyte.Mol Cell Proteomics. 2018 Oct;17(10):1875-1891.} {WB} {Buffalo}
Cadherin-Mediated Cell-Cell Interactions
P-Cadherin Human, mouse, bovine Placenta (mouse), skin, breast (myoepithelial cells), prostate (basal cells) R-Cadherin (cadherin
Testing Rabbit Bleeds By Elisa
at 1/10 (50 ul/well). Remove primary antibody and rinse wells 4Xs with TBST. Incubate in secondary antibody (1/5000 Goat anti-rabbit conjugated to AP) diluted in Blocking Buffer for 1 hr at RT (50 ul/well). Remove secondary antibody
Assays of Proteoglycan and Collagen Degradation in Cultures of Rabbit Cartilage Explants
Cultures of cartilage explants have long been used to study the effects of modulators of extracellular matrix degradation. We present a simple and rapid assay system, based on culture of rabbit cartilage explants, which permits study
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