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- bs-2178R
- 2025年10月24日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-2178R |
| 英文名称 | SM22 Alpha Rabbit pAb |
| 中文名称 | 细胞骨架相关蛋白抗体 |
| 英文别名 | 22 kDa actin binding protein; 22 kDa actin-binding protein; Transgelin; DKFZp686P11128; Human 22kDa smooth muscle protein; Protein WS3-10; SM22 alpha; SM22; SM22-alpha; SMCC; Smooth muscle protein 22-alpha; TAGL_HUMAN; TAGLN; TAGLN1; transgelin; Transgelin variant 2; WS3 10; Protein WS3-10; WS3-10. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC/IF=1:100-500, IF=1:100-500, Flow-Cyt=1ug/test Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat (Chicken, Dog, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human SM22 Alpha |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 22 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Cardiovascular > Cardiovascular Markers > Cell Markers > Smooth Muscle Cells Cardiovascular > Heart > Contractility > Inotropics Epigenetics and Nuclear Signaling > Transcription > Domain Families > Zinc Finger Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Binding Proteins Signal Transduction > Signaling Pathway > Calcium Signaling |
| 亚细胞定位 | Cytoplasm (Probable). |
| 相似性 | Belongs to the calponin family.
Contains 1 calponin-like repeat. Contains 1 CH (calponin-homology) domain. |
| 功能 | Actin cross-linking/gelling protein. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {ICC/IF} | {1:100-500} |
| {IF} | {1:100-500} |
| {Flow-Cyt} | {1ug/test} |
Primary Antibody (green line): Rabbit Anti-SM22 Alpha antibody (bs-2178R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-PE
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with PBST for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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文献和实验[IF={{ 8.2 }}] {Yang Shuang. et al. Myeloid-derived growth factor suppresses VSMC dedifferentiation and attenuates postinjury neointimal formation in rats by activating S1PR2 and its downstream signaling. ACTA PHARMACOL SIN. 2023 Sep;:1-14} {IF,WB} {Rat}
[IF={{ 4.996 }}] {Xi, Yue. et al. Low expression of IGFBP4 and TAGLN accelerate the poor overall survival of osteosarcoma. SCI REP-UK. 2022 Jun;12(1):1-20} {IF} {Human, Mouse}
[IF={{ 4.315 }}] {Yuping Shi. et al. CKAP4 contributes to the progression of vascular calcification (VC) in chronic kidney disease (CKD) by modulating YAP phosphorylation and MMP2 expression. Cell Signal. 2022 Jan;:110270} {WB} {Human}
[IF={{ 4.222 }}] {Yi Song. et al. LncRNA SENCR overexpression attenuated the proliferation, migration and phenotypic switching of vascular smooth muscle cells in aortic dissection via the miR-206/myocardin axis. Nutr Metab Cardiovas. 2022 Mar;:} {IHC} {Human}
[IF={{ 2.37 }}] {Zhang, Mingming. et al. Both high glucose and phosphate overload promote senescence-associated calcification of vascular muscle cells. INT UROL NEPHROL. Int Urol Nephrol. 2022 Apr;:1-13} {IF} {Mouse}
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
on Unfixed Chromatin from Cells and Tissues to Analyze Histone Modifications. To distinguish between alleles at loci of interest in precipitated chromatin fractions, we use "hot-stop" PCR (Steps 1-22) or SSCP (Steps 23-37). See Uejima et al. (2000
Protocol for intracytoplasmic staining of cytokines for FACS analysis
analysis: anti-IL2 (S4B6-FITC; Pharmingen 18004A or home made conjugate) for FL1 anti-TNF-alpha (MP6-XT22-PE; Pharmingen 18135A) for FL2 anti-IFN-gamma (XMG1.2-FITC; Pharmingen 18114A or home made conjugate) for FL1 anti-IL4 (11B11-PE
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