Tissue/cell: Rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-yrosinase Polyclonal Antibody, Unconjugated(bs-0819R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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- bs-0819R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0819R |
| 英文名称 | Tyrosinase Rabbit pAb |
| 中文名称 | 酪氨酸酶抗体 |
| 英文别名 | LB24 AB; LB24-AB; Monophenol monooxygenase; SK29 AB; Tumor rejection antigen AB; LB24 AB; Monophenol monooxygenase; OCA1A; OCAIA; Oculocutaneous albinism IA; SK29 AB; SK29-AB; Tumor rejection antigen AB; TYR; tyrosinase(oculocutaneous albinism IA); TYRO_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Dog, Pig, Cow, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human Tyrosinase |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 56 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Melanosome membrane; Single-pass type I membrane protein. |
| 相似性 | Belongs to the tyrosinase family. |
| 功能 | This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Tyrosinase is the key enzyme for melanin,synthesis in mammalian melanocytes and has been considered to be a unique marker for the study of melanocyte differentiation. A cDNA library was constructed from poly(A)+ mRNA from mouse melanocytes and screened using anti- tyrosinase antiserum and oligonucleotide probes corresponding to amino acid sequence of tyrosinase. sequencing of some cDNA clones positive in these screenings gave a nucleotide sequence of 1838 nucleotides including a open reading frame of 1344 nucleotides that was found to correspond exactly to the amino acid sequence of the cyanogen bromide fragments of tyrosinase. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
Lane 1: Eye (Mouse) Lysate at 40 ug
Lane 2: Skin (Mouse) Lysate at 40 ug
Primary: Anti-Tyrosinase (bs-0819R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 80 kD
Lane 1: Eye (Mouse) Lysate at 40 ug
Lane 2: Skin (Mouse) Lysate at 40 ug
Primary: Anti-Tyrosinase (bs-0819R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 80 kD
Observed band size: 80 kD
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文献和实验该产品被引用文献
[IF={{ 4.868 }}] {Miao F et al. Intramelanocytic Acidification Plays a Role in the Antimelanogenic and Antioxidative Propertiesof Vitamin C and Its Derivatives. Oxid Med Cell Longev. 2019 May 12;2019:2084805.} {WB} {Human}
[IF={{ 4.563 }}] {Kumiko Kobayashi-Nakamura. et al. Rhamnazin suppresses melanosome transport by promoting the ubiquitin-mediated proteasomal degradation of melanophilin. J Dermatol Sci. 2021 Dec;:} {IF} {Mouse}
[IF={{ 4.191 }}] {Lee C. J. et al. Melanogenesis regulatory activity of the ethyl acetate fraction from Arctium lappa L. leaf on α-MSH–induced B16/F10 melanoma cells. Industrial Crops and Products,2019 138, 111581.} {WB} {Mouse}
[IF={{ 4.171 }}] {Han H et al. Anti-Melanogenic Effect of Ethanolic Extract of Sorghum bicolor on IBMX–Induced Melanogenesis in B16/F10 Melanoma Cells. Nutrients. 2020 Mar 20;12(3).} {WB} {mouse}
[IF={{ 4.067 }}] {Kwong SP et al. Identification of photodegraded derivatives of usnic acid with improved toxicity profile and UVA/UVB protection in normal human L02 hepatocytes and epidermal melanocytes. J Photochem Photobiol B. 2020 Feb 6;205:111814.} {WB} {Human}
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Tyrosinase Rabbit pAb(bs-0819R)-50ul/100ul/200ul
￥1180 - 2800
