Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-CLCN3/CLC-3 Polyclonal Antibody, Unconjugated(bs-6981R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
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- bs-6981R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6981R |
| 英文名称 | CLCN3 Rabbit pAb |
| 中文名称 | 氯离子通道蛋白3抗体 |
| 英文别名 | Chloride channel 3; Chloride channel protein 3; Chloride transporter ClC 3; Chloride transporter ClC-3; Chloride transporter ClC3; ClC 3; ClC-3; ClC3; CLCN 3; CLCN3; CLCN3_HUMAN; H(+)/Cl(-) exchange transporter 3. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Dog, Pig, Cow, Horse) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human CLCN3/CLC-3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 91 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Neurotransmission > Receptors / Channels > More Ion Channels Signal Transduction > Adapters > Transmembrane Signal Transduction > Metabolism > Plasma Membrane > Channels |
| 亚基 | Homo- or heterodimer. Isoform 2 interacts with GOPC, PDZK1 and SLC9A3R1/EBP50. |
| 亚细胞定位 | Isoform 1: Membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Cytoplasmic vesicle, secretory vesicle membrane; Multi-pass membrane protein. Isoform 2: Membrane; Multi-pass membrane protein. Early endosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. |
| 组织特异性 | Expressed primarily in tissues derived from neuroectoderm. Within the brain, its expression is particularly evident in the hippocampus, olfactory cortex, and olfactory bulb. Highly expressed in aortic and coronary vascular smooth muscle cells, and aortic endothelial cells. Also expressed in tracheal and alveolar epithelial cells, and intima and media of the pulmonary vessels. Expressed in bronchus and colon (at protein level). |
| 翻译后修饰 | N-glycosylated. |
| 相似性 | Belongs to the chloride channel (TC 2.A.49) family. ClC-3/CLCN3 subfamily. Contains 2 CBS domains. |
| 功能 | Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Mediates the exchange of chloride ions against protons. Functions as antiporter and contributes to the acidification of the endosome and synaptic vesicle lumen, and may thereby affect vesicle trafficking and exocytosis. May play an important role in neuronal cell function through regulation of membrane excitability by protein kinase C. It could help neuronal cells to establish short-term memory. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CLCN3) Polyclonal Antibody, Unconjugated (bs-6981R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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文献和实验该产品被引用文献
[IF={{ 3.454 }}] {Blair HC et al. Support of Bone Mineral Deposition by Regulation of pH.Am J Physiol Cell Physiol. 2018 Oct 1;315(4):C587-C597.} {IF} {Mouse}
[IF={{ 2.5 }}] {Irina L. Tourkova. et al. Chloride/proton antiporters ClC3 and ClC5 support bone formation in mice. Bone Reports. 2024 Apr;:101763} {IF} {Mouse}
[IF={{ 0 }}] {Larrouture, Quitterie C., et al. "Chloride–hydrogen antiporters ClC‐3 and ClC‐5 drive osteoblast mineralization and regulate fine‐structure bone patterning in vitro." Physiological Reports 3.11 (2015): e12607.} {WB} {="Human"}
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CLCN3 Rabbit pAb(bs-6981R)-50ul/100ul/200ul
￥1180 - 2800
