Tissue/cell: Rat adrenal gland; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADRB3 Polyclonal Antibody, Unconjugated(bs-1063R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-1063R
- 2025年10月24日
- 产品信息以Bioss网站为准
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产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-1063R |
| 英文名称 | ADRB3 Rabbit pAb |
| 中文名称 | β3肾上腺素能受体抗体 |
| 英文别名 | beta 3 Adrenergic Receptor; beta3-adrenergic receptor; beta3-AR; adrenergic; beta-3-receptor; ADR B3; ADRB 3; ADRB3R; Adrenergic beta 3 receptor; B3AR; beta 3 adrenoceptor; beta 3 adrenoreceptor; beta 3AR; B3AR; BETA3AR; ADRB3_HUMAN. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Rat (Mouse, Dog, Pig, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human ADRB3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 44 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Cell membrane; Multi-pass membrane protein. |
| 组织特异性 | Expressed mainly in adipose tissues. |
| 相似性 | Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB3 sub-subfamily. |
| 功能 | Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. Beta-3 is involved in the regulation of lipolysis and thermogenesis. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | beta 3 Adrenergic Receptor is located mainly in adipose tissue and is involved in the regulation of lipolysis and thermogenesis. Beta adrenergic receptors are involved in the epenephrine and norepinephrine-induced activation of adenylate cyclase through the action of G proteins. beta 3 Adrenergic Receptor expression has been documented in adipose tissues, heart, and in smooth muscle of digestive and urinary tract organs (bladder, colon, small intestine, stomach, ureter). Utilization of alternate promoters and/or 3-prime untranslated regions may result in tissue-specific regulation of the expression of this protein. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
K562(Human) Cell Lysate at 40 ug
Primary: Anti-beta 3 Adrenergic Receptor (bs-1063R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 54 kD
K562(Human) Cell Lysate at 40 ug
Primary: Anti-beta 3 Adrenergic Receptor (bs-1063R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 44 kD
Observed band size: 54 kD
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文献和实验该产品被引用文献
[IF={{ 6.208 }}] {Lina S. Farhoumand. et al. Blockade of ß-Adrenergic Receptors by Nebivolol Enables Tumor Control Potential for Uveal Melanoma in 3D Tumor Spheroids and 2D Cultures. INT J MOL SCI. 2023 Jan;24(6):5894} {FCM} {Human}
[IF={{ 4.757 }}] {Katarina Andelova. et al. Cardiac Cx43 Signaling Is Enhanced and TGF-β1/SMAD2/3 Suppressed in Response to Cold Acclimation and Modulated by Thyroid Status in Hairless SHRM. BIOMEDICINES. 2022 Jul;10(7):1707} {WB} {Rat}
[IF={{ 4.699 }}] {Pellegrino Lippiello. et al. Role of β3‐adrenergic receptor in the modulation of synaptic transmission and plasticity in mouse cerebellar cortex. J Neurosci Res. 2020 Nov;98(11):2263-2274} {IF} {Mouse}
[IF={{ 4.2 }}] {Wang Yu-Wan. et al. Long-term exposure to constant light disrupts intestinal stem cells through sympathoexcitation-induced Wnt5a signaling inhibition. GASTROENTEROL REP. 2025 May;13:} {IHC} {Rat}
[IF={{ 3.647 }}] {Clarissa Germano Barp et al. Perivascular adipose tissue phenotype and sepsis vascular dysfunction: Differential contribution of NO, ROS and beta 3-adrenergic receptor. Life Sci. 2020 Aug 1;254:117819.} {IF} {Rat}
相关实验
= NAL), appears to form upon oxidative cyclization of the nonfluorescent 2:1 lysine-HNE Michael adduct-Schiff base cross-link (Scheme 1). Polyclonal antibody (PAb) to the NAL-HNE fluorophore was raised in rabbit and found to be highly specific
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ADRB3 Rabbit pAb(bs-1063R)-50ul/100ul/200ul
￥1180 - 2800
