EIF2AK3 | PERK Rabbit pAb(bs-2469R)-50ul/100ul/200ul产品图
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EIF2AK3 | PERK Rabbit pAb(bs-2

469R)-50ul/100ul/200ul
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  • bs-2469R
  • 2026年10月13日
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    北京博奥森生物技术有限公司
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-2469R
    英文名称PERK Rabbit pAb
    中文名称蛋白激酶样内质网激酶抗体
    英文别名DKFZp781H1925; E2AK3_HUMAN; EC 2.7.11.1; EIF2AK3; Eukaryotic translation initiation factor 2 alpha kinase 3; Eukaryotic translation initiation factor 2-alpha kinase 3; Heme regulated EIF2 alpha kinase; HRI; HsPEK; Pancreatic eIF2 alpha kinase; Pancreatic eIF2-alpha kinase; PEK; PRKR like endoplasmic reticulum kinase; PRKR-like endoplasmic reticulum kinase; WRS.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2μg/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human PERK
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量122 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > DNA / RNA > Translation > Regulation

    Epigenetics and Nuclear Signaling > RNAi > Eukaryotic Initiation factors (eIF's)

    Metabolism > Types of disease > Diabetes

    Metabolism > Types of disease > Metabolic disorders

    Metabolism > Types of disease > Obesity

    亚基

    Forms dimers with HSPA5/BIP in resting cells (PubMed:21543844).
    Oligomerizes in ER-stressed cells (PubMed:10854322).
    Interacts with DNAJC3 (PubMed:12446838).
    Interacts with MFN2 (PubMed:23921556).
    Interacts with TMEM33 (By similarity).
    Interacts with PDIA6 (By similarity).
    Interacts with LACC1 (By similarity).

    亚细胞定位

    Endoplasmic reticulum membrane; Single-pass type I membrane protein.

    组织特异性

    Ubiquitous.

    翻译后修饰

    Autophosphorylated. Phosphorylated at Tyr-615 following endoplasmic reticulum stress, leading to activate its tyrosine-protein kinase activity. Dephosphorylated by PTPN1/TP1B, leading to inactivate its enzyme activity.
    Oligomerization of the N-terminal ER luminal domain by ER stress promotes PERK trans-autophosphorylation of the C-terminal cytoplasmic kinase domain at multiple residues including Thr-980 on the kinase activation loopBy Similarity1 Publication
    N-glycosylated.
    ADP-ribosylated by PARP16 upon ER stress, which increases kinase activity.

    相似性

    Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily.
    Contains 1 protein kinase domain.

    功能

    Metabolic-stress sensing protein kinase that phosphorylates the alpha subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2-alpha) in response to various stress conditions (PubMed:9930704, PubMed:11106749, PubMed:23921556).
    Key activator of the integrated stress response (ISR) required for adaptation to various stress, such as unfolded protein response (UPR) and low amino acid availability (PubMed:9930704, PubMed:11106749, PubMed:23921556).
    EIF2S1/eIF-2-alpha phosphorylation in response to stress converts EIF2S1/eIF-2-alpha in a global protein synthesis inhibitor, leading to a global attenuation of cap-dependent translation, while concomitantly initiating the preferential translation of ISR-specific mRNAs, such as the transcriptional activators ATF4 and QRICH1, and hence allowing ATF4- and QRICH1-mediated reprogramming (PubMed:11106749, PubMed:23921556).
    Serves as a critical effector of unfolded protein response (UPR)-induced G1 growth arrest due to the loss of cyclin-D1 (CCND1) (PubMed:11035797).
    Involved in control of mitochondrial morphology and function (PubMed:23921556).

    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The protein encoded by this gene phosphorylates the alpha subunit of eukaryotic translation-initiation factor 2 (EIF2), leading to its inactivation, and thus to a rapid reduction of translational initiation and repression of global protein synthesis. It is a type I membrane protein located in the endoplasmic reticulum (ER), where it is induced by ER stress caused by malfolded proteins. Mutations in this gene are associated with Wolcott-Rallison syndrome. [provided by RefSeq, Jan 2010].

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}
    {Flow-Cyt}{2μg/Test}

     

    产品细节图片1
    Tissue/cell: mouse stomach tissue; 4% P‌‌araformaldehyde-fixed and paraffin-embedded;
    _x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    _x000D_ Incubation: Anti-PERK Polyclonal Antibody, Unconjugated(bs-2469R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    _x000D_
    产品细节图片2
    Blank control: U-87MG(blue).
    Primary Antibody:Rabbit Anti-PERK antibody(bs-2469R), Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
    Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
    Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
    _x000D_ _x000D_ Protocol
    _x000D_ The cells were fixed with 2% P‌‌araformaldehyde (10 min) , then permeabilized with 90% ice-cold methanol for 30 min on ice. Primary antibody (bs-2469R,1μg /1x10^6 cells) were incubated for 30 min on the ice, followed by 1 X PBS containing 0.5% BSA + 1 0% goat serum (15 min) to block non-specific protein-protein interactions. Then the Goat Anti-rabbit IgG/PE antibody was added into the blocking buffer mentioned above to react with the primary antibody at 1/200 dilution for 30 min on ice. Acquisition of 20,000 events was performed._x000D_
    产品细节图片3
    P‌‌araformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PERK) Polyclonal Antibody, Unconjugated (bs-2469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining._x000D_ _x000D_
    产品细节图片4
    P‌‌araformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PERK) Polyclonal Antibody, Unconjugated (bs-2469R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    产品细节图片5
    Sample:
    _x000D_ Lane 1: Cerebrum (Mouse) Lysate at 40 ug
    _x000D_ Lane 2: Cerebrum (Rat) Lysate at 40 ug
    _x000D_ Primary: Anti-PERK (bs-2469R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 150 kD
    _x000D_ Observed band size: 145 kD
    产品细节图片6
    Sample:
    _x000D_ Hela(Human) Cell Lysate at 30 ug
    _x000D_ 293T(Human) Cell Lysate at 30 ug
    _x000D_ Primary: Anti-PERK (bs-2469R) at 1/1000 dilution
    _x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    _x000D_ Predicted band size: 122 kD
    _x000D_ Observed band size: 122 kD
    _x000D_
    产品细节图片7
    Blank control（black line）:K562.
    _x000D_ Primary Antibody (green line): Rabbit Anti-PERK antibody (bs-2469R)
    _x000D_ Dilution:2ug/Test;
    _x000D_ Secondary Antibody（white blue line）: Goat anti-rabbit IgG-AF488
    _x000D_ Dilution: 0.5ug/Test.
    _x000D_ Isotype control（orange line）: Normal Rabbit IgG
    _x000D_ Protocol
    _x000D_ The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃, The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

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    EIF2AK3 | PERK Rabbit pAb(bs-2469R)-50ul/100ul/200ul
    ￥1180 - 2800