METTL1 Rabbit pAb(bs-18814R)-50ul/100ul/200ul

METTL1 Rabbit pAb(bs-18814R)-5

0ul/100ul/200ul
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  • ¥1180 - 2800
  • Bioss已认证
  • bs-18814R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格:50ul产品价格:¥1180.0
    规格:100ul产品价格:¥1980.0
    规格:200ul产品价格:¥2800.0
    产品编号bs-18814R
    英文名称METTL1 Rabbit pAb
    中文名称甲基化样蛋白1抗体
    英文别名C12orf1; D1075 like gene product; FLJ95748; Methyltransferase like 1; Methyltransferase like protein 1; Methyltransferase-like protein 1; Mettl1; TRM8; TRMB_HUMAN; TRMT8; tRNA(guanine N(7) ) methyltransferase; tRNA(guanine(46)-N(7))-methyltransferase; tRNA(guanine-N(7)-)-methyltransferase; tRNA(m7G46) methyltransferase; tRNA(m7G46)-methyltransferase; YDL201w.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse (Rat, Pig, Cow, Horse, Rabbit, Sheep)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human METTL1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量31 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > DNA / RNA > RNA Processing

    Epigenetics and Nuclear Signaling > DNA / RNA > Translation > tRNAs

    亚细胞定位Nucleus.
    组织特异性Ubiquitous.
    翻译后修饰Phosphorylation at Ser-27 inactivates its catalytic activity but does not affect the interaction with WDR4.
    相似性Belongs to the methyltransferase superfamily. TrmB family.
    功能Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This gene is similar in sequence to the S. cerevisiae YDL201w gene. The gene product contains a conserved S-adenosylmethionine-binding motif and is inactivated by phosphorylation. Alternative splice variants encoding different protein isoforms have been described for this gene. A pseudogene has been identified on chromosome X. [provided by RefSeq, Jul 2008]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    METTL1 Rabbit pAb(bs-18814R)-5
    P‌‌araformaldehyde-fixed, paraffin embedded (Mouse pancreas); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (METTL1) Polyclonal Antibody, Unconjugated (bs-18814R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    METTL1 Rabbit pAb(bs-18814R)-5
    Sample:
    293T(Human) Cell Lysate at 30 ug
    Primary: Anti-METTL1 (bs-18814R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 31 kD
    Observed band size: 32 kD

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