Sample:
_x000D_
Lane 1: Human PANC-1 cell lysates
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Lane 2: Human K562 cell lysates
_x000D_
Primary: Anti-FOXL2 (bs-6633R) at 1/1000 dilution
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Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_
Predicted band size: 45-50 kDa
_x000D_
Observed band size: 55 kDa
_x000D_
_x000D_
- ￥1180 - 2800
- Bioss
- bs-6633R
- 2026年10月13日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-6633R |
| 英文名称 | FOXL2 Rabbit pAb |
| 中文名称 | 叉头蛋白L2抗体 |
| 英文别名 | blepharophimosis; Blepharophimosis epicanthus inversus and ptosis 1; Blepharophimosis epicanthus inversus and ptosis; BPES 1; BPES; BPES1; epicanthus inversus and ptosis 1; forkhead box L2; Forkhead box protein L2; forkhead transcription factor FOXL2; FOX L2; FOXL 2; FOXL2; FOXL2_HUMAN; PFRK; PINTO; POF 3; POF3. |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Rat, Dog, Pig, Cow, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human FOXL2 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 45-50 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Epigenetics and Nuclear Signaling > Transcription > Domain Families > Forkhead Box Epigenetics and Nuclear Signaling > Transcription > Domain Families > Forkhead Box > Other FOXes |
| 亚基 | Interacts with ESR1 (By similarity). Interacts with SMAD3 (By similarity). Interacts with DDX20. Interacts with UBE2I/UBC9. |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | In addition to its expression in the developing eyelid, it is transcribed very early in somatic cells of the developing gonad (before sex determination) and its expression persists in the follicular cells of the adult ovary. |
| 翻译后修饰 | Sumoylated by SUMO1; sumoylation is required for transcriptional repression activity. |
| 相似性 | Contains 1 fork-head DNA-binding domain. |
| 功能 | Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis throught transcriptional repression of the Sertoli cell-promoting gene SOX9 (By similarity). Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen (By similarity). Is a regulator of CYP19 expression (By similarity). Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element (By similarity). Is a transcriptional repressor of STAR. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
Sample:
_x000D_ A431 Cell (Human) Lysate at 30 ug
_x000D_ Primary: Anti- FOXL2 (bs-6633R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 45-50kD
_x000D_ Observed band size: 45kD
_x000D_ A431 Cell (Human) Lysate at 30 ug
_x000D_ Primary: Anti- FOXL2 (bs-6633R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 45-50kD
_x000D_ Observed band size: 45kD
Sample:
_x000D_ K562 Cell(Human)Lysate at 30 ug
_x000D_ Primary: Anti- FOXL2 (bs-6633R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 45-50kD
_x000D_ Observed band size: 50kD
_x000D_ K562 Cell(Human)Lysate at 30 ug
_x000D_ Primary: Anti- FOXL2 (bs-6633R)at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 45-50kD
_x000D_ Observed band size: 50kD
Paraformaldehyde-fixed, paraffin embedded (mouse ovary tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (FOXL2) Polyclonal Antibody, Unconjugated (bs-6633R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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文献和实验该产品被引用文献
[IF=2.852] Cai et al. Molecular mechanisms of enhancing porcine granulosa cell proliferation and function by treatment in vitro with anti-inhibin alpha subunit antibody. (2015) Reprod.Biol.Endocrinol. 13:26 WB Porcine
[IF=2.24] Liu, Xing-Long, et al. "FOXL2 suppresses proliferation, invasion and promotes apoptosis of cervical cancer cells."International Journal of Clinical and Experimental Pathology 7.4 (2014): 1534-1543. IHC ="Human"
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FOXL2 Rabbit pAb(bs-6633R)-50ul/100ul/200ul
￥1180 - 2800
