Sample:
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Lane 1: Mouse Testis tissue lysates
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Lane 2: Mouse Cerebrum tissue lysates
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Lane 3: Rat Testis tissue lysates
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Lane 4: Rat Cerebrum tissue lysates
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Lane 5: Human HepG2 cell lysates
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Lane 6: Human MDA-MB-231 cell lysates
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Lane 7: Human HeLa cell lysates
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Lane 8: Human MCF-7 cell lysates
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Lane 9: Human A431 cell lysates
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Primary: Anti-GOLGA1 (bs-13486R) at 1/1000 dilution
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Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
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Predicted band size: 88 kDa
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Observed band size: 100 kDa
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50ul/100ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 产品编号 | bs-13486R |
| 英文名称 | GOLGA1 Rabbit pAb |
| 中文名称 | 高尔基体蛋白97抗体 |
| 英文别名 | GOGA1_HUMAN; Golgin subfamily A member 1; Golgin 97; Golgin-97; Golgin97; |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse, Rat |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human GOLGA1 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 88 kDa |
| 实际分子量 | 90-100 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚基 | Binds RAB6A. |
| 亚细胞定位 | Golgi apparatus, Golgi stack membrane; Peripheral membrane protein. |
| 相似性 | Contains 1 GRIP domain. |
| 功能 | Probably involved in maintaining Golgi structure. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The GRIP family member, golgin 97, is a trans-Golgi network peripheral membrane protein with an extensive coiled-coil structure (67% alpha-helical content) and a C-terminal GRIP domain. Golgin 97 localizes exclusively on the cytoplasmic face of the Golgi and can form homodimers. Binding of golgin 97 to the Golgi membrane is mediated by the G protein family member, Arl1. Golgin 97 acts as an essential player to the cell in the form of a tethering molecule associating with tubulovesicular carriers during the trafficking from the trans-Golgi network to the recycling endosome and/or early endosome. During poxvirus infection, golgin 97 accumulates at the site of viral replication and is incorporated into virions. It associates with the insoluble fraction of the virus core protein, playing a significant role in virus replication and maturation of the virus membrane and core protein. Golgin 97 takes on a rod-like shape and, although it seemingly lacks a transmembrane domain, it protrudes from the surface of the virion envelope. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |

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文献和实验相关实验
Generation of Spatio‐Temporally Controlled Targeted Somatic Mutations in the Mouse
Syringe (1‐ml equipped with a 25‐G needle; Terumo, cat. no. BS‐01 H2516) Gas anesthesia station for rodents (TEM)
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GOLGA1 | Golgin-97 Rabbit pAb(bs-13486R)-50ul/100ul
¥1180 - 1980






