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XBP1 Rabbit pAb(bs-1668R)-50ul

/100ul/200ul
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  • ￥1180 - 2800
  • Bioss已认证
  • bs-1668R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    规格：200ul产品价格：￥2800.0
    产品编号bs-1668R
    英文名称XBP1 Rabbit pAb
    中文名称细胞核转录因子X盒结合蛋白抗体
    英文别名X box-binding protein-1; XBP-1; XBP2; Tax responsive element binding protein 5; TREB5; X box binding protein 2; XBP 1; XBP2; XBP1_HUMAN; X-box-binding protein 1, cytoplasmic form; X-box-binding protein 1, luminal form; X-box-binding protein 1 in isoform 2.
    产品应用WB=1:500-2000, ICC/IF=1:100-500, Flow-Cyt=1ug/test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse (Rat, Chicken, Cow)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human XBP-1
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量29/40 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Epigenetics and Nuclear Signaling > Transcription > Other factors

    Epigenetics and Nuclear Signaling > Transcription > Transcription Factors

    亚细胞定位Nucleus.
    相似性Belongs to the bZIP family.
    Contains 1 bZIP domain.
    功能Transcription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料This gene encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box. This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter. It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator. It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1). The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor. The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress. A pseudogene of XBP1 has been identified and localized to chromosome 5. [provided by RefSeq, Jul 2008]

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {ICC/IF}{1:100-500}
    {Flow-Cyt}{1ug/test}

     

    产品细节图片1
    HepG2 cell; 4% P‌‌araformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (XBP1) polyclonal Antibody, Unconjugated (bs-1668R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
    产品细节图片2
    U-937 cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at room temperature,and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with XBP1 Antibody(bs-1668R) at 1:500 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed.Cells stained with primary antibody (green), and isotype control (orange).
    产品细节图片3
    Sample:
    Lane 1: Mouse Skin tissue lysates
    Lane 2: Rat Skin tissue lysates
    Lane 3: Rat Testis tissue lysates
    Primary: Anti-XBP1 (bs-1668R) at 1/500 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 29/40 kDa
    Observed band size: 29/42 kDa

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    图标文献和实验
    该产品被引用文献

    [IF={{ 6.706 }}] {Elisa Martino. et al. Milk Exosomal miR-27b Worsen Endoplasmic Reticulum Stress Mediated Colorectal Cancer Cell Death. NUTRIENTS. 2022 Jan;14(23):5081} {WB} {Human}

    [IF={{ 5.878 }}] {Xueying Zhang. et al. Scutellarin ameliorates hepatic lipid accumulation by enhancing autophagy and suppressing IRE1α/XBP1 pathway. 2021 Dec 02} {WB} {Mouse ,Human}

    [IF={{ 5.793 }}] {Chunyue Wang. et al. Neuroprotective effects of verbascoside against Alzheimer’s disease via the relief of endoplasmic reticulum stress in Aβ-exposed U251 cells and APP/PS1 mice. J Neuroinflamm. 2020 Dec;17(1):1-16} {WB} {Human}

    [IF={{ 5.3 }}] {Wenjing Lu. et al. 1α,25(OH)2D3 improves 17β-estradiol secretion and potentially alleviates endoplasmic reticulum stress in muskrat granulosa cells. BIOCHEM PHARMACOL. 2024 Dec;:116696} {WB} {Muskrat}

    [IF={{ 4.784 }}] {Wang L et al. Radioprotective effect of Hohenbuehelia serotina polysaccharides through mediation of ER apoptosis pathway in vivo. Int J Biol Macromol. 2019 Apr 15;127:18-26.} {IHC} {Mouse}

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    文献支持
    XBP1 Rabbit pAb(bs-1668R)-50ul/100ul/200ul
    ￥1180 - 2800