Sample: Raji(Human) Cell Lysate at 40 ug
Primary: Anti-p-LIM kinase2(Ser291+Ser293) (bs-18259R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 72 kD
Observed band size: 72 kD
- ￥1180 - 2800
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- bs-18259R
- 2025年10月16日
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50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-18259R |
| 英文名称 | phospho- LIM kinase 2 (Ser293) Rabbit pAb |
| 中文名称 | 磷酸化单丝氨酸蛋白激酶2抗体 |
| 英文别名 | LIM kinase 2(phospho S293); p-LIM kinase 2(phospho S293); LIM domain kinase 2; LIMK 2; LIMK-2; LIMK2; LIMK2_HUMAN. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human (Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human LIM Kinase 1 around the phosphorylation site of Ser293） |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 72 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Signal Transduction > Protein Phosphorylation > Ser / Thr Kinases > Other Kinases |
| 亚细胞定位 | Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus. |
| 组织特异性 | Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed. |
| 翻译后修饰 | Phosphorylated on serine and/or threonine residues by ROCK1. |
| 相似性 | Belongs to the protein kinase superfamily.
TKL Ser/Thr protein kinase family. Contains 2 LIM zinc-binding domains. Contains 1 PDZ (DHR) domain. Contains 1 protein kinase domain. |
| 功能 | Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
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文献和实验相关实验
of the serine/threonine kinaseAkt, which will phosphorylate GSK-3 at specific serine residues(Ser21 in GSK-3a and Ser9 in GSK-3b), leading to the inactivationof GSK-3 kinase activity (10) . However, this phosphorylationand inactivation of GSK-3 by Akt
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
3.1 and 3.6 ). This will provide a “specific activity” measurement relative to total protein in the cell, and is themost accurate measure of total GSK3 activity, or2. Immunoblot using antibodies to phospho-Ser21 (GSK3a)or phospho-Ser9 (GSK3b) (see Sections 3.1 –
Scientists to Prof. Zheng-Tao Wang(No. 39825129).References Awad, M.M., Enslen, H., Boylan, J.M., Davis, R.J., Gruppuso, P.A.,2000. Growth regulation via p38 mitogen-activated protein kinase in developing liver. J. Biol. Chem. 275, 38716–38721. Bonni
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phospho- LIM kinase 2 (Ser293) Rabbit pAb(bs-18259R)-50ul/100ul/200ul
￥1180 - 2800
