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anti-GRP78

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  • ￥1180 - 2800
  • Bioss已认证
  • 北京
  • bs-1219R
  • 2026年05月21日
  • WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,
  • Rabbit
  • Human,Mouse,Rat,
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北京博奥森生物技术有限公司
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原：

      KLH conjugated synthetic peptide derived from human GRP78

    • 亚型：

      IgG

    • 形态：

      Lyophilized or Liquid

    • 保存条件：

      Store at -20 °C for one year.

    • 克隆性：

      单克隆

    • 标记物：

      无

    • 适应物种：

      Human,Mouse,Rat,

    • 保质期：

      1年

    • 目录编号：

      bs-1219R

    • 库存：

      999

    • 供应商：

      北京博奥森生物技术有限公司

    • 宿主：

      Rabbit

    • 应用范围：

      WB=1:500-2000,Elisa=1:500-1000,IHC-P=1:400-800,IHC-F=1:400-800,IF=1:100-500,

    • 浓度：

      1mg/ml

    • 抗体英文名：

      GRP78

    • 抗体名：

      葡萄糖调节蛋白78/热休克蛋白70蛋白5抗体

    • 规格：

      50ul/100ul/200ul

    规格：50ul产品价格：￥1180.00
    规格：100ul产品价格：￥1980.00
    规格：200ul产品价格：￥2800.00
    产品编号bs-1219R
    英文名称GRP78 Rabbit pAb
    中文名称葡萄糖调节蛋白78/热休克蛋白70蛋白5抗体
    英文别名BIP; HSPA5; GRP-78; glucose regulated protein 78; 78 kDa glucose regulated protein; BIP; Endoplasmic reticulum lumenal Ca2+ binding protein grp78; FLJ26106; Glucose Regulated Protein 78kDa; GRP 78; Heat Shock 70kDa Protein 5; HSPA 5; HSPA-5; Immunoglobulin Heavy Chain Binding Protein; MIF2; GRP78_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Rat (Mouse, Pig)
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human GRP78
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量78 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Tags & Cell Markers > Subcellular Markers > Organelles > ER

    亚细胞定位Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    相似性Belongs to the heat shock protein 70 family.
    功能Probably plays a role in facilitating the assembly of multimeric protein complexes inside the ER.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料The 78 kDa glucose regulated protein/BiP (GRP78) belongs to the family of ~70 kDa heat shock proteins (HSP 70). GRP78 is a resident protein of the endoplasmic reticulum (ER) and may associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins that are incorrectly folded, thus preventing their export from the ER lumen. GRP78 is a highly conserved protein that is essential for cell viability. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP78 and other resident ER proteins including glucose regulated protein 94 (GRP 94) and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:100-500}
    {IHC-F}{1:100-500}
    {IF}{1:100-500}

     

    产品细节图片1
    Tissue/cell: rat testis tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
    Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
    Incubation: Anti-GRP78 Polyclonal Antibody, Unconjugated(bs-1219R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
    产品细节图片2
    Sample:
    Lane 1: Spleen (Rat) Lysate at 40 ug
    Lane 2: Cerebrum (Rat) Lysate at 40 ug
    Lane 3: U-87MG (Human) Cell Lysate at 30 ug
    Lane 4: Hela (Human) Cell Lysate at 30 ug
    Lane 5: HepG2 (Human) Cell Lysate at 30 ug
    Lane 6: MDA-MB-231 (Human) Cell Lysate at 30 ug
    Lane 7: A431 (Human) Cell Lysate at 30 ug
    Primary: Anti-GRP78 (bs-1219R) at 1/1000 dilution
    Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
    Predicted band size: 78 kD
    Observed band size: 74 kD
    产品细节图片3
    Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GRP78) Polyclonal Antibody, Unconjugated (bs-1219R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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    图标文献和实验
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