Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-VIP Polyclonal Antibody, Unconjugated (bs-0077R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
- ￥1180 - 2800
- Bioss
- bs-0077R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-0077R |
| 英文名称 | VIP Rabbit pAb |
| 中文名称 | 血管活性肠肽抗体 |
| 英文别名 | Vasoactive Intestinal peptide; MGC13587; Peptide histidine methioninamide 27; PHM 27; PHM27; PHV42; VIP peptides precursor; PHI; Peptide Histidine Isoleucine; Vasoactive intestinal peptide; VIP_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Rat (Human, Mouse, Chicken, Dog, Pig, Cow, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human VIP |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 3.3/18 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Neuroscience > Neurotransmitter > Neuropeptides > Hormones |
| 亚细胞定位 | Secreted. |
| 相似性 | Belongs to the glucagon family. |
| 功能 | VIP causes vasodilation, lowers arterial blood pressure, stimulates myocardial contractility, increases glycogenolysis and relaxes the smooth muscle of trachea, stomach and gall bladder. PHM and PHV also cause vasodilation. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | VIP(Vasoactive Intestinal peptide) is a 28-amino acid peptide structurally related tosecretin. It was originally isolated from intestinal extracts and shown to be a potent vasodilator. Subsequent work demonstrated that VIP is very widely distributed in the peripheral and central nervous systems, and probably should not be considered a true GI hormone. A huge number of biological effects have been attributed to VIP. With respect to the digestive system, VIP seems to induce smooth muscle relaxation (lower esophageal sphincter, stomach, gallbladder), stimulate secretion of water into pancreatic juice and bile, and cause inhibition of gastric acid secretion and absorption from the intestinal lumen. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |
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文献和实验该产品被引用文献
[IF={{ 6.208 }}] {Zhiqiang Zhang. et al. Vasoactive Intestinal Peptide (VIP) Protects Nile Tilapia (Oreochromis niloticus) against Streptococcus agalatiae Infection. INT J MOL SCI. 2022 Jan;23(23):14895} {WB} {Fish}
[IF={{ 4.45 }}] {Wieck, Minna M., et al. "Human and murine tissue-engineered colon exhibit diverse neuronal subtypes and can be populated by enteric nervous system progenitor cells when donor colon is aganglionic." Tissue Engineering A (2015).} {IHC} {=""}
[IF={{ 3.8 }}] {Yunfei Li. et al. Hesperidin Facilitating Gastrointestinal Motility by “Gut-brain axis” and “SCF/C-Kit Signaling Pathways”. POULTRY SCI. 2024 Oct;:104390} {IHC} {Chicken}
[IF={{ 3.508 }}] {Wieck et al. Human and Murine Tissue-Engineered Colon Exhibit Diverse Neuronal Subtypes and Can Be Populated by Enteric Nervous System Progenitor Cells When Donor Colon Is Aganglionic. (2016) Tissue.Eng.Part.A. 22:53-64} {IHC} {Human}
[IF={{ 3.411 }}] {Liu Dan. et al. RSV Promotes Epithelial Neuroendocrine Phenotype Differentiation through NODAL Signaling Pathway. Biomed Res Int. 2021;2021:9956078} {IF} {human}
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VIP Rabbit pAb(bs-0077R)-50ul/100ul/200ul
￥1180 - 2800
