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CYCS | Cytochrome C Rabbit pAb

(bs-0013R)-50ul/100ul/200ul

价格￥1180 - 2800

品牌Bioss
货号bs-0013R
更新日期2026年10月13日

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规格：
50ul/100ul/200ul

规格：	50ul	产品价格：	￥1180.0
规格：	100ul	产品价格：	￥1980.0
规格：	200ul	产品价格：	￥2800.0

产品编号	bs-0013R
英文名称	Cytochrome C Rabbit pAb
中文名称	细胞色素C抗体
英文别名	CytC; CYC; CYCS; Cytochrome c somatic; HCS; CYC_HUMAN; Cytochrome-c; MSA06; THC4.
产品应用	WB=1:500-2000, ICC/IF=1:100-500, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Mouse, Rat, Human (Pig, Cow, Horse)
抗体来源	Rabbit
免疫原	KLH conjugated synthetic peptide derived from human Cytochrome C
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Polyclonal
理论分子量	12 kDa
实际分子量	14.4kDa
浓度	1mg/ml
储存液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域	Cancer > Cancer Metabolism > Metabolic signaling pathway > Integration of energy metabolism Cancer > Cell Death > Apoptosis > Apoptosis Markers > Cytochrome C Cancer > Cell Death > Apoptosis > Metabolism Cancer > Cell Death > Apoptosis > Mitochondrial Cancer > Invasion/microenvironment > Apoptosis > Cytochrome C Cardiovascular > Lipids / Lipoproteins > Lipid Metabolism > Cytochromes Cell Biology > Apoptosis > Mitochondrial Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Energy Metabolism Metabolism > Pathways and Processes > Metabolic signaling pathways > Energy transfer pathways > Integration of energy Metabolism > Pathways and Processes > Metabolic signaling pathways > Lipid and lipoprotein metabolism > Lipases Metabolism > Pathways and Processes > Metabolism processes > Apoptosis Metabolism > Pathways and Processes > Mitochondrial Metabolism > Cytochromes Metabolism > Pathways and Processes > Mitochondrial Metabolism > Mitochondrial markers Metabolism > Pathways and Processes > Mitochondrial Metabolism > Oxidative phosphorylation Signal Transduction > Metabolism > Energy Metabolism Signal Transduction > Metabolism > Mitochondrial
亚细胞定位	Mitochondrion intermembrane space. Note=Loosely associated with the inner membrane.
翻译后修饰	Binds 1 heme c group covalently per subunit. Phosphorylation at Tyr-49 and Tyr-98 both reduce by half the turnover in the reaction with cytochrome c oxidase, down-regulating mitochondrial respiration.
相似性	Belongs to the cytochrome c family.
功能	Electron carrier protein. The oxidized form of the cytochrome c heme group can accept an electron from the heme group of the cytochrome c1 subunit of cytochrome reductase. Cytochrome c then transfers this electron to the cytochrome oxidase complex, the final protein carrier in the mitochondrial electron-transport chain. Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	Cytochrome C is an electron transporting protein that resides within the intermembrane space of the mitochondria, where it plays a critical role in the process of oxidative phosphorylation and production of cellular ATP. An increasing amount of interest has been directed toward the role which cytocrome C has been demonstrated to play in apoptotic processes. Following exposure to apoptotic stimuli, cytochrome C is rapidly released from the mitochondria into the cytosol, an event which may be required for the completion of apoptosis in some systems. Cytosolic cytochrome C functions in the activation of caspase 3, an ICE family molecule that is a key effector of apoptosis.

应用	推荐稀释比例
{WB}	{1:500-2000}
{ICC/IF}	{1:100-500}
{IHC-P}	{1:100-500}
{IHC-F}	{1:100-500}
{IF}	{1:100-500}

P‌‌araformaldehyde-fixed, paraffin embedded Rat Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

P‌‌araformaldehyde-fixed, paraffin embedded Mouse Kidney; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

P‌‌araformaldehyde-fixed, paraffin embedded Rat Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

P‌‌araformaldehyde-fixed, paraffin embedded Mouse Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

P‌‌araformaldehyde-fixed, paraffin embedded Human Heart; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

P‌‌araformaldehyde-fixed, paraffin embedded Human Liver; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

P‌‌araformaldehyde-fixed, paraffin embedded Human Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

P‌‌araformaldehyde-fixed, paraffin embedded Human Cervical Cancer; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with Cytochrome C Polyclonal Antibody, Unconjugated (bs-0013R) at 1:200 overnight at 4°C, followed by conjugation to the bs-0295G-HRP and DAB (C-0010) staining.

25 ug total protein per lane of various lysates (see on figure) probed with Cytochrome C polyclonal antibody, unconjugated (bs-0013R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

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