Ki-67 Rabbit pAb(bs-2130R)-50ul/100ul
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Ki-67 Rabbit pAb(bs-2130R)-50u

l/100ul
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  • ￥1180 - 1980
  • Bioss已认证
  • bs-2130R
  • 2025年10月24日
  • 产品信息以Bioss网站为准
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北京博奥森生物技术有限公司
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      50ul/100ul

    规格：50ul产品价格：￥1180.0
    规格：100ul产品价格：￥1980.0
    产品编号bs-2130R
    英文名称Ki-67 Rabbit pAb
    中文名称Ki67抗体
    英文别名Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN.
    产品应用WB=1:500-2000, IHC-P=1:200-800, IHC-F=1:200-800, ICC/IF=1:100-500, IF=1:200-800, Flow-Cyt=1ug/Test

    Not yet tested in other applications.
    Optimal working dilutions must be determined by the end user.

    交叉反应Human, Mouse, Rat
    抗体来源Rabbit
    免疫原KLH conjugated synthetic peptide derived from human Ki67
    亚型IgG
    性状Liquid
    纯化方法affinity purified by Protein A
    克隆类型Polyclonal
    理论分子量358 kDa
    实际分子量320/359 kDa
    浓度1mg/ml
    储存液0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
    研究领域

    Cancer > Cell cycle > Cell division

    Cancer > Tumor biomarkers

    Cell Biology > Cell Cycle > Markers

    Neuroscience > Cell Type Marker > Neuron marker > Soma marker

    Neuroscience > Neurology process > Neurogenesis

    Tags & Cell Markers > Cell Type Markers > Replication

    亚细胞定位Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
    相似性Contains 1 FHA domain.
    功能Thought to be required for maintaining cell proliferation.
    保存条件Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
    注意事项This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
    背景资料Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

     

    应用推荐稀释比例
    {WB}{1:500-2000}
    {IHC-P}{1:200-800}
    {IHC-F}{1:200-800}
    {ICC/IF}{1:100-500}
    {IF}{1:200-800}
    {Flow-Cyt}{1ug/Test}

     

    Ki-67 Rabbit pAb(bs-2130R)-50u
    4% P‌‌araformaldehyde-fixed Hela(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Ki-67) polyclonal Antibody, unconjugated (bs-2130R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.
    Ki-67 Rabbit pAb(bs-2130R)-50u
    P‌‌araformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Ki-67 Rabbit pAb(bs-2130R)-50u
    P‌‌araformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
    Ki-67 Rabbit pAb(bs-2130R)-50u
    P‌‌araformaldehyde-fixed, paraffin embedded Human ovarian serous carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (bs-2130R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.
    Ki-67 Rabbit pAb(bs-2130R)-50u
    P‌‌araformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (bs-2130R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

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    图标文献和实验
    该产品被引用文献

    [IF={{ 9.995 }}] {Qihang Hou. et al. Bacillus subtilis programs the differentiation of intestinal secretory lineages to inhibit Salmonella infection. CELL REP. 2022 Sep;40:111416} {IF} {Mouse}

    [IF={{ 9.417 }}] {Zhenyin Chen. et al. Uninterrupted dynamic stiffening microenvironment enhances the paracrine function of mesenchymal stem cells for vascularization through chromatin remodeling. MATER DESIGN. 2022 Dec;224:111328} {IF} {Human}

    [IF={{ 8.6 }}] {Dongzhou Wang. et al. A multifunctional bimetallic nanoplatform for enhancing radiotherapy via oxidative damage and ferroptosis of cervical cancer. SEP PURIF TECHNOL. 2024 Jun;338:126567} {IHC} {Mouse}

    [IF={{ 8.355 }}] {Chen Z et al. Enzyme-powered Janus nanomotors launched from intratumoral depots to address drug delivery barriers. Chemical Engineering Journal,2019 375, 122109.} {IHC} {Mouse}

    [IF={{ 8.355 }}] {Xie S et al. Bacteria-propelled microrockets to promote the tumor accumulation and intracellular drug uptake. Chemical Engineering Journal,2019, 123786.} {IHC} {Mouse}

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    文献支持
    Ki-67 Rabbit pAb(bs-2130R)-50ul/100ul
    ￥1180 - 1980