文献支持

MKI67 | Ki-67 Rabbit pAb(bs-21

30R)-50ul/100ul

价格￥1180 - 1980

品牌Bioss
货号bs-2130R
更新日期2026年10月13日

10 年钻石会员

详细信息
文献和实验
技术资料

规格：
50ul/100ul

规格：	50ul	产品价格：	￥1180.0
规格：	100ul	产品价格：	￥1980.0

产品编号	bs-2130R
英文名称	Ki-67 Rabbit pAb
中文名称	Ki67抗体
英文别名	Antigen identified by monoclonal antibody Ki 67; Antigen KI67; KIA; Ki67; MKI67; Proliferation related Ki 67 antigen; Antigen KI-67; KI67_HUMAN.
产品应用	WB=1:500-2000, IHC-P=1:200-800, IHC-F=1:200-800, ICC/IF=1:100-500, IF=1:200-800, Flow-Cyt=1ug/Test Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Human, Mouse, Rat
抗体来源	Rabbit
免疫原	KLH conjugated synthetic peptide derived from human Ki67
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Polyclonal
理论分子量	358 kDa
实际分子量	320/359 kDa
浓度	1mg/ml
储存液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域	Cancer > Cell cycle > Cell division Cancer > Tumor biomarkers Cell Biology > Cell Cycle > Markers Neuroscience > Cell Type Marker > Neuron marker > Soma marker Neuroscience > Neurology process > Neurogenesis Tags & Cell Markers > Cell Type Markers > Replication
亚细胞定位	Nucleus. Chromosome. Predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.
相似性	Contains 1 FHA domain.
功能	Thought to be required for maintaining cell proliferation.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	Ki67 antigen is the prototypic cell cycle related nuclear protein, expressed by proliferating cells in all phases of the active cell cycle (G1, S, G2 and M phase). It is absent in resting (G0) cells. Ki67 antibodies are useful in establishing the cell growing fraction in neoplasms (immunohistochemically quantified by determining the number of Ki67 positive cells among the total number of resting cells = Ki67 index). In neoplastic tissues the prognostic value is comparable to the tritiated thymidine labelling index. The correlation between low Ki67 index and histologically low grade tumours is strong. Ki67 is routinely used as a neuronal marker of cell cycling and proliferation.

应用	推荐稀释比例
{WB}	{1:500-2000}
{IHC-P}	{1:200-800}
{IHC-F}	{1:200-800}
{ICC/IF}	{1:100-500}
{IF}	{1:200-800}
{Flow-Cyt}	{1ug/Test}

4% P‌‌araformaldehyde-fixed Hela(H) cell; Triton X-100 at r.t. for 20 min; Antibody incubation with (Ki-67) polyclonal Antibody, unconjugated (bs-2130R) 1:100, 90 min at 37°C; followed by conjugated Goat Anti-Rabbit IgG antibody (green, bs-0295G-FITC) at 37°C for 90 min, DAPI (blue, C02-04002) was used to stain the cell nuclei. PBS instead of the primary antibody was used as the blank control.

P‌‌araformaldehyde-fixed, paraffin embedded (human lung carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

P‌‌araformaldehyde-fixed, paraffin embedded (Human stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ki-67) Polyclonal Antibody, Unconjugated (bs-2130R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

P‌‌araformaldehyde-fixed, paraffin embedded Human ovarian serous carcinoma; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (bs-2130R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

P‌‌araformaldehyde-fixed, paraffin embedded Human Tonsil; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with Ki-67 Polyclonal Antibody, Unconjugated (bs-2130R) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit, SP-0023) and DAB (C-0010) staining.

风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。