Protein: bladder(mouse) lyates at 40ug;
Primary: Rabbit Anti- p-GATA3 (Ser308) (bs-13294R) at 1:300;
Secondary: 800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L) at 1: 10000;
Predicted band size:49 kD
Observed band size:49 kD
- ￥1180 - 2800
- Bioss
- bs-13294R
- 2025年10月16日
- 产品信息以Bioss网站为准
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- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-13294R |
| 英文名称 | phospho-GATA3 (Ser308) Rabbit pAb |
| 中文名称 | 磷酸化GATA结合蛋白3抗体 |
| 英文别名 | GATA3(phospho S308); p-GATA3(phospho S308); GATA3(phospho Ser308); p-GATA3(Ser308); GATA 3; GATA3; GATA-3; GATA binding factor 3; GATA binding protein 3; HDR; MGC2346; MGC5199; MGC5445; Trans acting T cell specific transcription factor GATA 3; GATA3_HUMAN; Trans-acting T-cell-specific transcription factor GATA-3; GATA-binding factor 3. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Human, Mouse (Dog, Cow, Rabbit, Sheep, GuineaPig) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthesised phosphopeptide derived from human GATA3 around the phosphorylation site of Ser308 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 49 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Developmental Biology > Lineage specification > Ectoderm Epigenetics and Nuclear Signaling > ChIP assays > ChIP antibodies Epigenetics and Nuclear Signaling > Transcription > Domain Families > Zinc Finger Immunology > Adaptive Immunity > T Cells > Non-CD Stem Cells > Lineage Markers > Ectoderm |
| 亚细胞定位 | Nucleus. |
| 组织特异性 | T-cells and endothelial cells. |
| 相似性 | Contains 2 GATA-type zinc fingers. |
| 功能 | Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | Members of the GATA family share a conserved zinc finger DNA-binding domain and are capable of binding the WGATAR consensus sequence. GATA-1 is erythroid-specific and is responsible for the regulated transcription of erythroid genes. It is an essential component in the generation of the erythroid lineage. GATA-2 is expressed in embryonic brain and liver, HeLa and endothelial cells, as well as in erythroid cells. Studies with a modified GATA consensus sequence, AGATCTTA, have shown that GATA-2 and GATA-3 recognize this mutated consensus while GATA-1 has poor recognition of this sequence. This indicates broader regulatory capabilities of GATA-2 and GATA-3 than GATA-1. GATA-3 is highly expressed in T lymphocytes. GATA-4, GATA-5 and GATA-6 comprise a subfamily of transcription factors. Both GATA-4 and GATA-6 are found in heart, pancreas and ovary; lung and liver tissues exhibit GATA-6, but not GATA-4 expression. GATA-5 expression has been observed in differentiated heart and gut tissues and is present throughout the course of development in the heart. Although expression patterns of the various GATA transcription factors may overlap, it is not yet apparent how the GATA factors are able to discriminate in binding their appropriate target sites. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
Sample:
Lane 1: Human SH-SY5Y cell lysates
Lane 2: Human A431 cell lysates
Primary: Anti-phospho-GATA3 (Ser308) (bs-13294R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
Lane 1: Human SH-SY5Y cell lysates
Lane 2: Human A431 cell lysates
Primary: Anti-phospho-GATA3 (Ser308) (bs-13294R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 49 kDa
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：使用 Anti-phospho-Akt (Ser473) Rabbit mAb 对石蜡包埋的人乳腺癌组织进行免疫组织化学分析。（图 A）使用免疫组化试剂盒M&R HRP/DAB Detection IHC Kit，抗体 1:100 稀释；(图 B) 采用普通免疫组化试剂盒，抗体 1:25 稀释。 图 6 免疫组化实验检测 Erk1/2 表达 注：使用 Anti-Erk1/2 Mouse mAb与p44/42 MAPK （Erk1/2）Rabbit mAb 对正常小鼠心脏组织进行免疫
at 10, 25, and 50 μM). At 24 h post-treatment, celllysates were obtained; SDS-PAGE and Western blotting were performed as described in Section 3, Methods. AR-A014418dose-dependent decreases of phospho-β-catenin (Ser33/37) and phospho-glycogen synthase
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phospho-GATA3 (Ser308) Rabbit pAb(bs-13294R)-50ul/100ul/200ul
￥1180 - 2800
