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- 技术资料
- 规格:
50ul/100ul/200ul
| 规格: | 50ul | 产品价格: | ¥1180.0 |
|---|---|---|---|
| 规格: | 100ul | 产品价格: | ¥1980.0 |
| 规格: | 200ul | 产品价格: | ¥2800.0 |
| 产品编号 | bs-13118R |
| 英文名称 | EXOC2 Rabbit pAb |
| 中文名称 | 胞吐囊复合体蛋白2抗体 |
| 英文别名 | exocyst complex component 2; exocyst complex component Sec5; FLJ11026; SEC5; SEC5 like 1; SEC5L1; Sec5p; EXOC2_HUMAN. |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. |
| 交叉反应 | Mouse, Rat (Human, Chicken, Pig, Cow, Horse, Rabbit) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human EXOC2/SEC5 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 104 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 研究领域 | Signal Transduction > Protein Trafficking > Vesicle Transport > Regulation |
| 亚基 | The exocyst complex is composed of EXOC1, EXOC2, EXOC3, EXOC4, EXOC5, EXOC6, EXOC7 and EXOC8. Interacts with EXOC3L1 (By similarity). Interacts with RALA and GNEFR/DELGEF. Interaction with GNEFR occurs only in the presence of magnesium or manganese and is stimulated by dCTP or GTP. Interacts with RALB. |
| 亚细胞定位 | Plasma membrane. |
| 组织特异性 | Widely expressed with highest levels in brain and placenta. |
| 相似性 | Belongs to the SEC5 family. Contains 1 IPT/TIG domain._x000D_ |
| 功能 | Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The mammalian exocyst complex (sec 6/8) is a multiple protein complex essential for targeting exocytic vesicles to specific docking sites on the plasma membrane. Sec 5 is one of eight exocyst complex subunits: Sec3, Sec5, Sec6, Sec8, Sec10, Sec15, Exo70, and Exo84. Exocyst complex regulation depends upon Sec5 binding Ral in a GTP-dependent manner. Human Sec5 maps to chromosome 6p25.3. |
| 应用 | 推荐稀释比例 |
| {IHC-P} | {1:100-500} |
| {IHC-F} | {1:100-500} |
| {IF} | {1:100-500} |

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (EXOC2) Polyclonal Antibody, Unconjugated (bs-13118R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-EXOC2 Polyclonal Antibody, Unconjugated(bs-13118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_ _x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-EXOC2 Polyclonal Antibody, Unconjugated(bs-13118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_ _x000D_

Tissue/cell: mouse embryo tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-EXOC2 Polyclonal Antibody, Unconjugated(bs-13118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_ _x000D_
_x000D_ Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
_x000D_ Incubation: Anti-EXOC2 Polyclonal Antibody, Unconjugated(bs-13118R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
_x000D_ _x000D_
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EXOC2 | SEC5 Rabbit pAb(bs-13118R)-50ul/100ul/200ul
¥1180 - 2800










