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ACTA1 | alpha-Actin-1 Rabbit p

Ab(bs-10966R)-50ul/100ul/200ul

价格￥1180 - 2800

品牌Bioss
货号bs-10966R
更新日期2026年10月13日

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规格：
50ul/100ul/200ul

规格：	50ul	产品价格：	￥1180.0
规格：	100ul	产品价格：	￥1980.0
规格：	200ul	产品价格：	￥2800.0

产品编号	bs-10966R
英文名称	Actin Rabbit pAb
中文名称	肌动蛋白α1抗体
英文别名	muscle Actin; ACTS_HUMAN; Actin, alpha skeletal muscle; alpha-actin-1; ACTA1; ACTA; ASMA; CFTD; CFTD1; CFTDM; MPFD; NEM1; NEM2; NEM3; Actin alpha skeletal muscle; actin, alpha 1, skeletal muscle 1; actin, alpha 1, skeletal muscle; actina; aktin; alpha Actin 1; alpha skeletal muscle Actin; alpha skeletal muscle; alpha-actin; beta cytoskeletal actin; nemaline myopathy type 3.
中文别名	α-skeletal muscle actin; α Skeletal Muscle Actin.
产品应用	WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500 Not yet tested in other applications. Optimal working dilutions must be determined by the end user.
交叉反应	Mouse, Rat (Human, Dog, Cow, Rabbit)
抗体来源	Rabbit
免疫原	KLH conjugated synthetic peptide derived from human ACTA1
亚型	IgG
性状	Liquid
纯化方法	affinity purified by Protein A
克隆类型	Polyclonal
理论分子量	42 kDa
浓度	1mg/ml
储存液	0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
研究领域	Cancer > Cell Death > Apoptosis > Receptors > Death receptors & ligands > RIP Cancer > Invasion/microenvironment > Apoptosis > Death receptors & ligands > RIP Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Assembly Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Binding Proteins Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Actin Crosslinking Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Tropomyosin Signal Transduction > Cytoskeleton / ECM > Cytoskeleton > Microfilaments > Actin etc > Troponin Tags & Cell Markers > Subcellular Markers > Cytoskeleton > Actin
亚基	Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a complex composed of ACTA1, COBL, GSN AND TMSB4X. Interacts with TTID. Interacts (via its C-terminus) with USP25; the interaction occurs for all USP25 isoforms but is strongest for isoform USP25min muscle differentiating cells.
亚细胞定位	Cytoplasm, cytoskeleton.
翻译后修饰	Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promote actin repolymerization._x000D_ Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration.
相似性	Belongs to the actin family.
功能	Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
保存条件	Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
背景资料	The product encoded by this gene belongs to the actin family of proteins, which are highly conserved proteins that play a role in cell motility, structure and integrity. Alpha, beta and gamma actin isoforms have been identified, with alpha actins being a major constituent of the contractile apparatus, while beta and gamma actins are involved in the regulation of cell motility. This actin is an alpha actin that is found in skeletal muscle. Mutations in this gene cause nemaline myopathy type 3, congenital myopathy with excess of thin myofilaments, congenital myopathy with cores, and congenital myopathy with fiber-type disproportion, diseases that lead to muscle fiber defects. [provided by RefSeq, Jul 2008]

应用	推荐稀释比例
{WB}	{1:500-2000}
{IHC-P}	{1:100-500}
{IHC-F}	{1:100-500}
{IF}	{1:100-500}

P‌‌araformaldehyde-fixed, paraffin embedded (mouse skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Actin) Polyclonal Antibody, Unconjugated (bs-10966R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

Sample:
_x000D_ Bone (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-Actin (bs-10966R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 42 kD
_x000D_ Observed band size: 42 kD

Sample:
_x000D_ Heart (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-Actin (bs-10966R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 42 kD
_x000D_ Observed band size: 42 kD

Sample:
_x000D_ Muscle (Mouse) Lysate at 40 ug
_x000D_ Primary: Anti-Actin (bs-10966R) at 1/300 dilution
_x000D_ Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
_x000D_ Predicted band size: 42 kD
_x000D_ Observed band size: 42 kD

P‌‌araformaldehyde-fixed, paraffin embedded (rat skeletal muscle); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Actin) Polyclonal Antibody, Unconjugated (bs-10966R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.