Sample:
Bone (Mouse) Lysate at 40 ug
Primary: Anti- MYLK3 (bs-9865R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 98 kD
Observed band size: 100 kD
- ￥1180 - 2800
- Bioss
- bs-9865R
- 2025年10月24日
- 产品信息以Bioss网站为准
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- 技术资料
- 应用范围：
产品信息以Bioss网站为准
- 规格：
50ul/100ul/200ul
| 规格： | 50ul | 产品价格： | ￥1180.0 |
|---|---|---|---|
| 规格： | 100ul | 产品价格： | ￥1980.0 |
| 规格： | 200ul | 产品价格： | ￥2800.0 |
| 产品编号 | bs-9865R |
| 英文名称 | MYLK3 Rabbit pAb |
| 中文名称 | 肌球蛋白轻链激酶3抗体 |
| 英文别名 | caMLCK; Cardiac MyBP C associated Ca/CaM kinase; Cardiac-MyBP-C-associated Ca/CaM kinase; MLC kinase; MLCK; MLCK2; Mylk3; MYLK3_HUMAN; Myosin light chain kinase 3; Putative myosin light chain kinase 3. |
| 产品应用 | WB=1:500-2000 Not yet tested in other applications. |
| 交叉反应 | Mouse (Human, Rat, Dog, Cow, Horse, Rabbit, Sheep) |
| 抗体来源 | Rabbit |
| 免疫原 | KLH conjugated synthetic peptide derived from human MYLK3 |
| 亚型 | IgG |
| 性状 | Liquid |
| 纯化方法 | affinity purified by Protein A |
| 克隆类型 | Polyclonal |
| 理论分子量 | 98 kDa |
| 浓度 | 1mg/ml |
| 储存液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 亚细胞定位 | Cytoplasm. |
| 组织特异性 | May play a role in smooth muscle contraction. |
| 翻译后修饰 | Phosphorylated on serine residues (By similarity). |
| 相似性 | Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain. |
| 功能 | Kinase that phosphorylates MYL2 in vitro. Promotes sarcomere formation in cardiomyocytes and increases cardiomyocyte contractility (By similarity). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 背景资料 | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) are a structurally related subfamily of serine/threonine kinases that includes CaMKI, CaMKII, CaMKIV and Myosin light chain kinases (MYLKs, also designated MLCKs). The MYLK kinases phosphorylate Myosin regulatory light chains to catalyze Myosin interaction with Actin filaments, resulting in contractile activity. MYLK3 (Myosin light chain kinase 3) is a 795 amino acid cardiac-specific protein that contains one protein kinase domain and belongs to the protein kinase superfamily. Like other MYLK kinases, MYLK3 is thought to play a role in smooth muscle contraction, specifically using magnesium as a cofactor to catalyze the ATP-dependent phosphorylation of Myosin light chain proteins. Additionally, MYLK3 may regulate sarcomere assembly in heart tissue, possibly mediating proper heart function. |
| 应用 | 推荐稀释比例 |
| {WB} | {1:500-2000} |
25 ug total protein per lane of various lysates (see on figure) probed with MYLK3 polyclonal antibody, unconjugated (bs-9865R) at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.
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MYLK3 Rabbit pAb(bs-9865R)-50ul/100ul/200ul
￥1180 - 2800
